GST Ubiquitin Mutt K63R
    Mutation of lysine 63 to arginine renders ubiquitin (Ub) unable to form poly‐Ub chains via lysine 63 linkages with
other Ub molecules. UBM GK63R with N‐terminal GST‐tag (glutathione S‐trsferase). GST‐Ub allows for glutathione
affinity purification of ubiquitinated molecules d allows for immuno‐ detection of conjugates using GST
tibodies. UBM GK63R c form E1‐catalyzed active thioester at the C‐terminus allowing the molecule to be
trsferred to the lysines of substrate proteins. Ideal for the reduction in poly‐Ub chain length/conjugation rates d
determining if poly‐Ub chains are K63 linked