This ready-to-use digital PCR supermix for probes is ideal for use with uracil N-glucosylase (UNG) decontamination protocols to prevent the reamplification of carryover PCR products between experiments.
描述
This digital PCR supermix for probes is a 2x concentrated, ready-to-use universal mix that has been optimized to deliver maximum PCR efficiency, specificity, and sensitivity in Droplet Digital PCR (ddPCR).
This supermix is suitable for use with UNG decontamination protocols.
Key Benefits
- Contains all components required for hydrolysis probe–based ddPCR except primers, probe(s), and template
- Limits nonspecific PCR amplification
- Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
Applications and Uses
- Absolute quantification
- Mutation detection
- Copy number analysis
- Gene expression analysis
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
|---|---|---|
| 200 | 2 x 1 | 186-3026 |
| 500 | 5 x 1 | 186-3010 |
| 2,500 | 5 x 5 | 186-3027 |
| 5,000 | 10 x 5 | 186-3028 |
Additional Reagents
Bio-Rad offers additional consumables for completing your digital PCR workflow:
- Droplet Generation Oil for Probes
- ddPCR Droplet Reader Oil
- DG8 Cartridges
- DG8 Gaskets
Additional Equipment
These digital PCR assays require the use of the following instruments and accessories:
- QX200 Droplet Generator
- QX200 Droplet Reader
- PX1 PCR Plate Sealer
- C1000 Touch Thermal Cycler with 96 Deep–Well Reaction Module
More Information
The ddPCR Supermix for Probes (no dUTP) is optimized for use with Droplet Generation Oil for Probes on the QX200 Droplet Digital PCR System.
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1863026
1863010
1863027
1863028
配套设备
1863005
1863052
1863004
2 L (2 x 1 L), oil for use with droplet reader in the QX200™/QX100™ Droplet Digital™ PCR Systems
1864008
1863009
1864007
1814040
Equipment
1864001
Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
1864002
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
1864003
1814000
1851197
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive
相关资料
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Droplet Digital™ PCR (ddPCR™) Supermixes Flier, Rev A
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ddPCR™ Supermix for Probes Product Insert
Droplet Generation Oil for Probes Product Insert
QX200 Droplet Digital PCR System Brochure, Rev C
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Instruction Manual, QX200™ Droplet Generator, Rev C
Instruction Manual, QX200 Droplet Reader and QuantaSoft Software