伯乐Nuvia™ IMAC Metal Affinity Resin | Bio-Rad Laboratories

Nuvia IMAC (immobilized metal affinity chromatography) Resin provides an ultra-high capacity matrix over a wide range of flow rates and pH for process scale chromatography. Get reproducible separation of biomolecules free of contaminants.

描述

Nuvia IMAC Resin is a metal chelate affinity chromatography resin that contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The inert hydrophilic surface of the base bead and the chemical structure of NTA ensure highly selective binding of recombinant histidine-tagged proteins when charged with Ni²⁺, or with other transition metals such as Zn²⁺ or Cu²⁺. The optimized particle size and high porosity allows operation at increasingly higher flow rates without compromising binding capacity. Nuvia IMAC can be used at any scale from laboratory to manufacturing, providing robust, reproducible, and efficient purifications each time.

Nuvia IMAC Resin is stable in a broad range of chemicals. It is compatible with all reagents traditionally used for histidine-tagged protein purification, including reducing agents, detergents, denaturing agents, and additives.

Features and Benefits

Easy and efficient scale up from lab to manufacturing scale purifications
Chelating ligand nitrilotriacetic acid (NTA) may be charged with transition metals such as Cu²⁺, Ni²⁺, Co²⁺, or Zn²⁺
Optimized particle size for high flow rate and low backpressure
Superior dynamic binding capacity at high flow rates
Withstands repeated clean-in-place/recharging cycles
Robust recovery and reproducibility
Full regulatory support

Applications and Uses of Nuvia IMAC Resin

For purifying biomolecules in a variety of workflows:

Excellent single-step purity via affinity chromatography
Quick, efficient separation of biomolecules
Contaminant removal
Robust base matrix

Nuvia IMAC Resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.

Product Options

Package Size	Catalog Number
Nuvia IMAC Ni-Charged Support, bulk resin
25 ml	7800800
100 ml	7800801
500 ml	7800802
5 L	12003233
10 L	12002782
Nuvia IMAC Ni-Charged Support, prepacked
Prepacked column, 1 ml	12004038
Prepacked column, 5 ml	12004037
Prepacked filter plates, 20 µl	12004035
Prepacked RoboColumns, 200 µl	12004051
Prepacked RoboColumns, 600 µl	12004052
Nuvia IMAC Uncharged Support, bulk resin
5 L	12004040
10 L	12004039

More Information

Download the Nuvia IMAC Resin Instruction Manual for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage. Prepacked Bio-Scale™ Mini Nuvia IMAC Cartridges with Ni-charged NTA are available.

技术指标

Particle size

38–53 µm

Ligand density

≥ 18 µmol/ml

Dynamic binding capacity*

> 40 mg/ml at 300 cm/hr
*10% breakthrough capacity determined with 1.2 mg/ml of a 40 kD histidine-tagged protein in 50 mM sodium phosphate, 5 mM imidazole, 300 mM NaCl (pH 7.5).

Compression factor

1.20–1.25

Recommended linear flow rate range

50–300 cm/hr

Pressure vs. flow performance

Under 2 bar at flow rate of 300 cm/hr in DI water (20 x 20 cm packed bed, 1.2 compression factor)

PH 稳定性

2–14

Shipping solution

2% benzyl alcohol or 20% ethanol

再生

50 mM EDTA, pH 8.0 (Stripping)
1N NaOH (CIP/SIP)
0.1 M Ni₂SO₄ (Recharging)

就地清洁 (CIP) 解决方案

1N NaOH

Sanitization

1N NaOH

Storage condition

20% Ethanol

化学兼容性

Reducing agents (β-ME, TCEP, DTT)
Denaturing agents (GnHCl)
Detergents (Triton X-100, NP-40, CHAPS, CHAPSO)
Additives (glycerol)
**No recharge required. Refer to the instruction manual for a complete list.

化学稳定性

48 hr with 1M NaOH, 1 week with 0.01M HCl
***Recharge required. Refer to the instruction manual for a complete list.

订购

items

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(停产) Nuvia™ IMAC Resin, 25 ml

7800800

Pkg of 1, 25 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

(停产) Nuvia™ IMAC Resin, 100 ml

7800801

Pkg of 1, 100 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

(停产) Nuvia™ IMAC Resin, 500 ml

7800802

Pkg of 1, 500 ml, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

(停产) Nuvia™ IMAC Resin, 5 L

12003233

Pkg of 1, 5 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

(停产) Nuvia™ IMAC Resin, 10 L

12002782

Pkg of 1, 10 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography

(停产) Nuvia™ IMAC Uncharged Resin, 5 L

12004040

Pkg of 1, 5 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography

(停产) Nuvia™ IMAC Uncharged Resin, 10 L

12004039

Pkg of 1, 10 L, Nuvia IMAC Support with uncharged nitrilotriacetic acid (NTA) for immobilized metal affinity chromatography

(停产) Foresight™ Nuvia™ Ni-Charged IMAC, 1 ml column

12004038

Pkg of 1, 1 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin

(停产) Foresight™ Nuvia™ Ni-Charged IMAC, 5 ml column

12004037

Pkg of 1, 5 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin

(停产) Foresight™ Nuvia™ Ni-Charged IMAC Filter Plates, 20 µl

12004035

2 x 96-well plates, 20 µl, filter plate prepacked with Nuvia IMAC Affinity Chromatography Resin

(停产) Foresight™ Nuvia™ Ni-Charged IMAC RoboColumn Unit, 200 µl

12004051

1 row of 8 columns, 200 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin

(停产) Foresight™ Nuvia™ Ni-Charged IMAC RoboColumn Unit, 600 µl

12004052

1 row of 8 columns, 600 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin

(停产) Bio-Scale™ Mini Nuvia™ IMAC Cartridges

7800811

Pkg of 1, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system

The following replacement is recommended:

EconoFit Nuvia IMAC Column, Ni-charged, 1 x 5 ml ( 12009286 )

(停产) Bio-Scale™ Mini Nuvia™ IMAC Cartridges

7800812

Pkg of 5, 5 ml, prepacked Ni-charged column, 40 x 12.6 mm, max. pressure 45 psi, for use with NGC™ or BioLogic™ Systems or any low- or medium-pressure chromatography system