![DEAE Affi-Gel Blue 凝胶 | Bio-Rad Laboratories](http://www.swablab.com/wp-content/uploads/2021/12/20211226_61c82ff0d0de2.jpg)
DEAE Affi-Gel Blue 凝胶是一种将 Cibacron Blue F3GA 和溴化甲基基团偶联到 Bio-Gel A 而形成的双功能亲和/离子交换层析基质。
描述
DEAE Affi-Gel 蓝胶是一种双功能亲和凝胶,与 DEAE Bio-Gel A 相同,含有共价结合的 Cibacron blue F3GA染料。Cibacron blue F3GA可作为离子、疏水或蛋白质空间活性的结合位点(如白蛋白)。溴化甲基基团可作为阴离子交换基团,结合等电点高于溶液pH的蛋白质。利用这种双功能的特性,可以通过优化缓冲液pH和离子强度从动物血清中获得高纯度的IgG。
主要功能
- 一步纯化血清中 IgG,IgG 洗脱液中含有少量转铁蛋白(样品大约稀释 5 倍)
- IgG 洗脱液中无蛋白水解活性
- Protein A 亲和层析经济的替代品
Related Technologies
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Affinity Chromatography -
Activated Supports -
Introduction to Chromatography
Related Products
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Econo-Pac® 层析柱 -
Bio-Scale™ Mini DEAE Affi-Gel® 蓝胶预装柱 -
Econo-Pac® 蛋白 A 预装重力流柱
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![DEAE Affi-Gel Blue 凝胶 | Bio-Rad Laboratories](http://www.swablab.com/wp-content/uploads/2021/12/20211226_61c82ff136c2c.jpg)
(停产) DEAE Affi-Gel Blue Gel
1537307
100 ml, ready-to-use bifunctional affinity gel with Cibacron Blue F3GA dye covalently coupled to DEAE Bio-Gel A
![DEAE Affi-Gel Blue 凝胶 | Bio-Rad Laboratories](http://www.swablab.com/wp-content/uploads/2021/12/20211226_61c82ff18334e.png)
(停产) EconoFit DEAE Affi-Gel Blue Column, 1 x 5 ml
12009262
Pkg of 1, 5 ml, prepacked affinity column, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system
![DEAE Affi-Gel Blue 凝胶 | Bio-Rad Laboratories](http://www.swablab.com/wp-content/uploads/2021/12/20211226_61c82ff230227.png)
(停产) EconoFit DEAE Affi-Gel Blue Columns, 5 x 5 ml
12009263
Pkg of 5, 5 ml, prepacked affinity columns, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system