伯乐DEAE Affi-Gel Blue 凝胶 | Bio-Rad Laboratories

DEAE Affi-Gel Blue 凝胶 | Bio-Rad Laboratories

DEAE Affi-Gel Blue 凝胶是一种将 Cibacron Blue F3GA 和溴化甲基基团偶联到 Bio-Gel A 而形成的双功能亲和/离子交换层析基质。

描述

DEAE Affi-Gel 蓝胶是一种双功能亲和凝胶,与 DEAE Bio-Gel A 相同,含有共价结合的 Cibacron blue F3GA染料。Cibacron blue F3GA可作为离子、疏水或蛋白质空间活性的结合位点(如白蛋白)。溴化甲基基团可作为阴离子交换基团,结合等电点高于溶液pH的蛋白质。利用这种双功能的特性,可以通过优化缓冲液pH和离子强度从动物血清中获得高纯度的IgG。

主要功能

  • 一步纯化血清中 IgG,IgG 洗脱液中含有少量转铁蛋白(样品大约稀释 5 倍)
  • IgG 洗脱液中无蛋白水解活性
  • Protein A 亲和层析经济的替代品

 

Related Technologies

  • Affinity Chromatography

  • Activated Supports

  • Introduction to Chromatography

Related Products

  • Econo-Pac® 层析柱

  • Bio-Scale™ Mini DEAE Affi-Gel® 蓝胶预装柱

  • Econo-Pac® 蛋白 A 预装重力流柱

订购

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DEAE Affi-Gel Blue 凝胶 | Bio-Rad Laboratories

(停产) DEAE Affi-Gel Blue Gel

1537307

100 ml, ready-to-use bifunctional affinity gel with Cibacron Blue F3GA dye covalently coupled to DEAE Bio-Gel A

DEAE Affi-Gel Blue 凝胶 | Bio-Rad Laboratories

(停产) EconoFit DEAE Affi-Gel Blue Column, 1 x 5 ml

12009262

Pkg of 1, 5 ml, prepacked affinity column, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system

DEAE Affi-Gel Blue 凝胶 | Bio-Rad Laboratories

(停产) EconoFit DEAE Affi-Gel Blue Columns, 5 x 5 ml

12009263

Pkg of 5, 5 ml, prepacked affinity columns, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system