CHEF 染色体 DNA 插块试剂盒提供了一种方便的方法来为 PFGE 制备完整的染色体规模 DNA。
描述
CHEF 染色体 DNA 插块试剂盒提供了一种方便的方法来为 PFGE 制备完整的染色体规模 DNA。 对于这种大分子，吸液会以机械方式剪切 DNA，导致 PFGE 分离质量不合格。 此问题要求对琼脂糖中嵌入的全细胞执行裂解和脱蛋白程序以生成完整的纯化 DNA。 提供三种试剂盒用于制备细菌（溶菌酶敏感型）或哺乳动物染色体 DNA 和酵母菌染色体 (YACs)。 每个包含有所有的酶、反应缓冲液和准备 100 个插块所需的限制性可消化 CleanCut 琼脂糖、用于简化插块处理的一次性插块模具和屏蔽帽。 每个包都进行了彻底的测试，以确保可以在 CHEF 电泳系统上限制性消化和分离准备好的染色体 DNA。
Related Technologies
Pulsed Field Gel Electrophoresis
CHEF Solutions
脉冲场电泳系统
Related Products
CHEF-DR® III 可变角系统
脉冲电场标准品和琼脂糖
CHEF Mapper® XA 系统
CHEF-DR II 系统
技术指标
CHEF 哺乳动物染色体 DNA 插块试剂盒
细胞悬浮液缓冲液
12 ml
>600 U/ml 蛋白酶 K
1.3 ml
蛋白酶 K 反应缓冲液
30 ml
2% CleanCut™ 琼脂糖
12 ml
10x 清洗缓冲液
60 ml
50 孔一次性插块模具
2
屏蔽帽
1
CHEF 细菌染色体 DNA 插块试剂盒
Cell suspension buffer
12 ml
>600 U/ml proteinase K
1.3 ml
Proteinase K reaction buffer
30 ml
2% CleanCut agarose
12 ml
10x wash buffer
60 ml
25 mg/ml 溶菌酶
1.6 ml
溶菌酶缓冲液
30 ml
50-well disposable plug mold
2
Screened cap
1
CHEF 酵母菌染色体 DNA 插块试剂盒
Cell suspension buffer
12 ml
>600 U/ml proteinase K
1.3 ml
Proteinase K reaction buffer
30 ml
2% CleanCut agarose
12 ml
10x wash buffer
60 ml
5,000 U/ml 溶细胞酶
1.6 ml
溶细胞酶缓冲液
30 ml
50-well disposable plug mold
2
屏蔽帽
1
订购
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(停产) CHEF Mammalian Genomic DNA Plug Kit
1703591
Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, all required buffers, screened cap, 2 plug molds, for 100 plugs
(停产) CHEF Bacterial Genomic DNA Plug Kit
1703592
Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs
(停产) CHEF Yeast Genomic DNA Plug Kit
1703593
Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs
(停产) CleanCut Agarose
1703594
12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs
系统
(停产) CHEF Mapper XA Chiller System, 120 V
1703670
120 V, complete PFGE system, includes cooling module, pump, tubing, S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose
(停产) CHEF-DR III Chiller System
1703700
120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of S. cerevisiae DNA size standard, PFGE grade agarose
The following replacement is recommended:
CHEF Mapper XA Chiller System, 120 V ( 1703670 )
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) CHEF-DR II Chiller System
1703725
120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose
The following replacement is recommended:
CHEF Mapper XA Chiller System, 120 V ( 1703670 )
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
相关资料
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Number描述Options
1753
Preparation and Restriction Digestion of Escherichia coli Chromosomal DNA in Agarose Plugs for Use in PFGE Bulletin, Rev A
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LIT510
Instruction Manual, CHEF Genomic DNA Plug Kit, Rev E
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5901
Pulsed Field Gel Electrophoresis Brochure, Rev B
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6228
Using Pulsed Field Gel Electrophoresis Standards, Rev A
