The 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D electrophoresis results.
描述
Bio-Rad’s 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D results.
4 Steps
- Sample Preparation — sample preparation is the first step toward optimizing your results. Bio-Rad offers easy-to-use kits: ReadyPrep™ 2-D starter kit, ReadyPrep 2-D cleanup kit, and ProteoMiner™ protein enrichment kit for getting started and for cleanup and enrichment of low-abundance proteins.
- First Dimension Electrophoresis — the first dimension of 2-D electrophoresis is separation based on a protein’s isoelectric point. Bio-Rad’s IPG strips offer many pH gradient options and strict quality control. The award-winning PROTEAN® i12™ IEF system gives you the flexibility to run multiple pH gradients, protocols, and samples simultaneously.
- Second Dimension Electrophoresis — the second dimension of 2-D electrophoresis is separation based on molecular weight. Bio-Rad’s Criterion™ system, Criterion precast gels, Criterion™ Dodeca™ cell, and Precision Plus Protein™ standard plugs provide the confidence of a leakproof precast gel and the ability to run up to 12 gels at once.
- Staining, Imaging, and Analysis — staining, imaging, and analysis allow you to document and evaluate your 2-D results. With Bio-Rad’s sensitive and hassle-free Oriole™ gel stain and versatile ChemiDoc™ MP imager, you can obtain high-quality images. Analyze those images with PDQuest™ software, a highly powerful tool for 2-D evaluation.
Applications and Technical Resources
The 2-D electrophoresis applications and technology page is a virtual encyclopedia of 2-D electrophoresis. You’ll find extensive information, videos, and troubleshooting guides to ensure you get the best 2-D results possible.
On the 2-D Electrophoresis applications and technology page you can:
- See a wide range of protein sample preparation information including protocols and videos to help guide you through the very important sample preparation step
- Learn all about first-dimension separation, including IEF, IPG strips, pH gradient ranges, frequently asked questions, how-to videos, and protocols
- Be guided through the 2-D instrument selection process on the second-dimension separation page
- Visit the 2-D Doctor™ page, which is a valuable 2-D self-help guide that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges
- Discover the best way to stain and visualize your proteins after 2-D Electrophoresis
- View imaging and analysis of 2-D gels
- Explore protein identification and protein spot excision
Related Technologies
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Protein Sample Preparation for 2-D Electrophoresis -
2D Protein Spot Excision & Detection -
Isoelectric Focusing in 2-D Electrophoresis -
2-D Electrophoresis -
Second-Dimension Separation -
Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor -
Staining and Visualization of Proteins After 2-D Electrophoresis -
Imaging and Analysis of 2-D Electrophoresis Gels
Related Products
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ReadyPrep™ 2-D Cleanup Kit -
PROTEAN® i12™ IEF 水化/聚焦盘 -
ReadyStrip™ IPG 胶条 -
PDQuest 双向电泳分析软件 -
ProteoMiner 蛋白质浓缩试剂盒 -
EXQuest 点切割器
订购
items
Use the filters below to refine results!
1646000
1708280
The following replacement is recommended:
-
ChemiDoc™ Imaging System ( 12003153 ) -
ChemiDoc™ MP Imaging System ( 12003154 )
1654139
1709630
配套设备
3459920
1708289
试剂&试剂盒
1610378
1610496
1633006
1633007
1632130
1632105
Gels
5671071
5671081
5671091
5671101
5671111
The following replacement is recommended:
-
AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl ( 5671121 )
5671121
IPG Strips
1632014
1632015
1632016
1632017
1632018
1632019
1632024
1632025
1632026
The following replacement is recommended:
-
ReadyStrip™ IPG Strips ( 1632018 )
1632027
相关资料
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2-D Electrophoresis Workflow Brochure, Rev B
[ Add to Cart (Free) ]
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
[ Add to Cart (Free) ]
2-D Electrophoresis Workflow How-To Guide, Rev F
ProteoMiner Protein Enrichment Technology Bulletin, Rev B
Instruction Manual, ProteoMiner Protein Enrichment Kits, Rev D
Instruction Manual, ReadyPrep 2-D Cleanup Kit, Rev A
Instruction Manual, ReadyPrep 2-D Starter Kit, Rev A
ReadyPrep 2-D Starter Kit Product Information Sheet
PROTEAN i12 IEF System Brochure, Ver B
[ Add to Cart (Free) ]
PROTEAN i12 IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions, Rev A
Instruction Manual, ReadyStrip IPG Strip
ReadyStrip IPG Strip Product Information Sheet, Rev F
Instruction Manual, Criterion Dodeca Cell, Rev A
Electrophoresis Guide, Interactive PDF, Rev C
Instruction Manual, Criterion™ Gel Application Guide, Ver H
Oriole Fluorescent Gel Stain: A Novel Fluorescent Stain for a Simpler 2-D PAGE to MALDI Workflow, Rev A
Oriole Fluorescent Gel Stain PIS, Rev A
Bio-Rad Stain-Free Western Blotting
[ Add to Cart (Free) ]
PDQuest 2-D Analysis Software Brochure, Rev C
EXQuest Spot Cutter Brochure, Rev A
Instruction Manual, EXQuest™ Spot Cutter, Rev A
Electrophoresis Power Supplies Brochure, Rev C
[ Add to Cart (Free) ]
Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins, Rev A
[ Add to Cart (Free) ]
Effectiveness of 2-D Electrophoresis over 1-D Electrophoresis Followed by Western Blotting for Evaluating Strategies Used to Generate Host Cell Protein Antibodies, Rev A