伯乐2-D Electrophoresis Workflow | Bio-Rad Laboratories

2-D Electrophoresis Workflow | Bio-Rad Laboratories

The 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D electrophoresis results.

描述

Bio-Rad’s 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D results.

4 Steps

  1. Sample Preparation — sample preparation is the first step toward optimizing your results. Bio-Rad offers easy-to-use kits: ReadyPrep™ 2-D starter kit, ReadyPrep 2-D cleanup kit, and ProteoMiner™ protein enrichment kit for getting started and for cleanup and enrichment of low-abundance proteins.
  2. First Dimension Electrophoresis — the first dimension of 2-D electrophoresis is separation based on a protein’s isoelectric point. Bio-Rad’s IPG strips offer many pH gradient options and strict quality control. The award-winning PROTEAN® i12™ IEF system gives you the flexibility to run multiple pH gradients, protocols, and samples simultaneously.
  3. Second Dimension Electrophoresis — the second dimension of 2-D electrophoresis is separation based on molecular weight. Bio-Rad’s Criterion™ system, Criterion precast gels, Criterion™ Dodeca™ cell, and Precision Plus Protein™ standard plugs provide the confidence of a leakproof precast gel and the ability to run up to 12 gels at once.
  4. Staining, Imaging, and Analysis — staining, imaging, and analysis allow you to document and evaluate your 2-D results. With Bio-Rad’s sensitive and hassle-free Oriole™ gel stain and versatile ChemiDoc™ MP imager, you can obtain high-quality images. Analyze those images with PDQuest™ software, a highly powerful tool for 2-D evaluation.

2-D Electrophoresis Workflow | Bio-Rad Laboratories

Applications and Technical Resources

The 2-D electrophoresis applications and technology page is a virtual encyclopedia of 2-D electrophoresis. You’ll find extensive information, videos, and troubleshooting guides to ensure you get the best 2-D results possible.

On the 2-D Electrophoresis applications and technology page you can:

  • See a wide range of protein sample preparation information including protocols and videos to help guide you through the very important sample preparation step
  • Learn all about first-dimension separation, including IEF, IPG strips, pH gradient ranges, frequently asked questions, how-to videos, and protocols
  • Be guided through the 2-D instrument selection process on the second-dimension separation page
  • Visit the 2-D Doctor™ page, which is a valuable 2-D self-help guide that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges
  • Discover the best way to stain and visualize your proteins after 2-D Electrophoresis
  • View imaging and analysis of 2-D gels
  • Explore protein identification and protein spot excision

Related Technologies

  • Protein Sample Preparation for 2-D Electrophoresis

  • 2D Protein Spot Excision & Detection

  • Isoelectric Focusing in 2-D Electrophoresis

  • 2-D Electrophoresis

  • Second-Dimension Separation

  • Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor

  • Staining and Visualization of Proteins After 2-D Electrophoresis

  • Imaging and Analysis of 2-D Electrophoresis Gels

Related Products

  • ReadyPrep™ 2-D Cleanup Kit

  • PROTEAN® i12™ IEF 水化/聚焦盘

  • ReadyStrip™ IPG 胶条

  • PDQuest 双向电泳分析软件

  • ProteoMiner 蛋白质浓缩试剂盒

  • EXQuest 点切割器

订购

items

Use the filters below to refine results!

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) PROTEAN® i12™ IEF system

1646000

Isoelectric focusing system, includes basic unit, electrode assemblies, focusing and rehydration/equilibration trays, forceps, electrode wicks, mineral oil, cleaning accessories, IPG strips and sample/rehydration buffer, USB flash drives, styluses

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ChemiDoc™ MP System

1708280

This model of the ChemiDoc MP Imaging System has been replaced by the new ChemiDoc MP Imager.

The following replacement is recommended:

  • ChemiDoc™ Imaging System ( 12003153 )
  • ChemiDoc™ MP Imaging System ( 12003154 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) Criterion™ Dodeca™ Cell and PowerPac™ Universal Power Supply

1654139

Vertical midi-format electrophoresis cell (#165-4130), 12 gel capacity, includes buffer tank with cooling coil, lid with power cables, universal power supply (#164-5070)

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) PDQuest Advanced 2-D Analysis Software

1709630

1-user license, 2-D analysis software, provides advanced functionality

配套设备

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) Criterion Staining/Blotting Trays

3459920

Pkg of 12, gel staining trays, with lids

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) White Light Conversion Screen

1708289

Pkg of 1, conversion screen for viewing Coomassie, silver stain, and other colorimetric gel stains; for use with non touch–screen ChemiDoc™ MP, ChemiDoc™ XRS+, and Gel Doc™ XR+ Systems

试剂&试剂盒

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) Precision Plus Protein™ Standard Plugs, unstained, Strep-tagged, recombinant

1610378

Pkg of 24, 1 mm thick agarose plugs containing ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) Oriole Fluorescent Gel Stain

1610496

1 L, 1x Oriole fluorescent gel stain solution

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ProteoMiner™ Protein Enrichment Small-Capacity Kit

1633006

10 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ProteoMiner™ Protein Enrichment Large-Capacity Kit

1633007

10 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyPrep™ 2-D Cleanup Kit for IEF, 50 preps

1632130

50 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 50 x 100 µl protein samples each containing up to 500 µg protein

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyPrep™ 2-D Starter Kit

1632105

2-D gel electrophoresis kit, includes protein sample and buffers for IPG strip rehydration, focusing, and transfer to second dimension (IPG strips not included)

Gels

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) 18% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671071

Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) 4–15% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671081

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) 4–20% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671091

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671101

Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) 10–20% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671111

Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

The following replacement is recommended:

  • AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl ( 5671121 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) AnykD™ Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671121

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

IPG Strips

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632014

Pkg of 12, 11 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632015

Pkg of 12, 11 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632016

Pkg of 12, 11 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632017

Pkg of 12, 11 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632018

Pkg of 12, 11 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632019

Pkg of 12, 11 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632024

Pkg of 12, 11 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632025

Pkg of 12, 11 cm, pH 4.7–5.9, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632026

Pkg of 12, 11 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

The following replacement is recommended:

  • ReadyStrip™ IPG Strips ( 1632018 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

2-D Electrophoresis Workflow | Bio-Rad Laboratories

(停产) ReadyStrip™ IPG Strips

1632027

Pkg of 12, 11 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

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