伯乐CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

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CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

使用 CFX96 Touch Deep Well 实时 PCR 检测系统，可在大容量反应（最大125 ul）中进行精确的定量和靶标识别。

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CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

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CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

实时 PCR 的提示、技巧和最佳实践指南

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CFX96 Touch Deep Well 实时 PCR 检测系统可在大体积反应中 对多达 5 个靶标 进行精准的定量和靶标区分。该系统结合了业界领先的技术和单机运行功能，是功能强大且易于使用的 qPCR 仪，可获得稳定可靠的结果。

主要特点和优点

CFX96 Touch Deep Well 实时 PCR 检测系统可提供：

  • 工厂荧光染料校正，简化实验设置
  • 集成 LCD 触摸屏可轻松查看实时数据轨迹
  • 内置温度梯度 可快速优化实验
  • CFX Maestro 软件具有 用户友好的数据分析模块
  • 轻松导出 RDML 文件以导入到 qbasePLUS 软件中

Related Technologies

  • Introduction to qPCR Instrumentation

  • What is Real-Time PCR (qPCR)?

  • MIQE and RDML Guidelines

  • Mutational Analysis

  • What is Gene Expression Analysis?

  • Real-Time PCR Experimental Design

  • Genomics

  • Analysis of Stem Cells

Related Products

  • iScript™ Reverse Transcription Supermix for RT-qPCR

  • T100™ PCR 仪

  • 测试产品

  • Precision Melt Analysis™ 软件

  • C1000 Touch PCR 仪

技术指标

PCR 仪
 

Chassis
C1000 Touch™

最大升降温速率，°C/sec
2.5

平均升降温速率，°C/sec
2

加热和冷却方法
Peltier

盖子， °C
可加热至高达 105

温度
 

范围，°C
0–100

精度：°C
90°C 时，编程设定靶标 ±0.2

均匀性：°C
到达 90°C 时，10 秒内孔间温差 ±0.4

梯度
 

工作范围，°C
30–100

可编程范围，°C
1–24

光学检测
 

激励
6 个滤光 LED

检测
6 个滤波光电二极管

激发/发射波长的范围，nm
450–730

灵敏度
可检测到人类基因组 DNA 中靶序列的 1 个拷贝

动态范围
10 个数量级

扫描时间
 

所有通道，秒
12

FAM/SYBR® Green only, sec
3

软件
 

操作系统
Windows XP, Windows 7, Windows 10

多重分析
每孔最多 5 个靶标

系统
 

已针对荧光定量 PCR 进行许可
是

样本载量，孔
96

样本尺寸，µl
10–125

通信接口
USB 2.0

电气认证
IEC, CE

Dimensions (W x D x H), cm/in
33 x 46 x 36/13 x 18 x 14

Weight, kg/lb
21/47

配套设备

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) 1000-Series Connectivity Kit

1841001

Kit contains mouse, mouse pad, USB flash drive

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) CFX Qualification Plate

1845098

Predispensed 96-well plate for performance validation of CFX96™, CFX96 Touch™, CFX96 Touch™ Deep Well, or CFX Connect™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water

试剂&试剂盒

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725270

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725271

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced Universal SYBR® Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725272

10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 1,000 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced Universal SYBR® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725274

25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 2,500 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725275

50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 5,000 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced Universal Probes Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725280

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 200 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725281

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725282

10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 1,000 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced Universal Probes Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725284

25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 2,500 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) SsoAdvanced Universal Probes Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725285

50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 5,000 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725120

200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725121

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal SYBR® Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725122

1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal SYBR® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725124

2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725125

5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, and a blend of passive reference dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal Probes Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725130

200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725131

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725132

1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal Probes Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725134

2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal Probes Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725135

5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal SYBR® Green One-Step Kit, 100 x 20 µl rxns, 1 ml

1725150

100 x 20 µl reactions, 1 ml (1 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq™ Universal SYBR® Green One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725151

500 x 20 µl reactions, 5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq Universal Probes One-Step Kit, 100 x 20 µl rxns, 1 ml

1725140

100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iTaq Universal Probes One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725141

500 x 20 µl reactions, 5 ml (5 x 1 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 200 x 20 µl reactions, 2 x 1 ml

1725110

2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 1,000 x 20 µl reactions, 10 x 1 ml

1725112

10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iQ™ Multiplex Powermix, 50 x 50 µl rxns, 1.25 ml

1725848

50 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix contains dNTPs, 11 mM MgCl2, iTaq™ DNA polymerase

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iQ™ Multiplex Powermix, 200 x 50 µl rxns, 5 ml (4 x 1.25 ml)

1725849

200 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix contains dNTPs, 11 mM MgCl2, iTaq™ DNA polymerase

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns

1708896

25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iScript™ Select cDNA Synthesis Kit, 100 x 20 µl rxns

1708897

100 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iScript™ Select cDNA Synthesis Kit, 500 x 20 µl rxns

1708897BUN

500 x 20 µl reactions, includes 5x reaction mix, iScript Reverse Transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) iScript™ Reverse Transcription Supermix, 25 x 20 µl rxns, 100 µl

1708840

25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

塑料耗材

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear

HSP9601

Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear, barcoded

HSP9901

Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design

The following replacement is recommended:

  • Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white ( HSP9655 )
本产品不再供货。欲了解更多关于更换产品的信息，请联系移动电话

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655

Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) Multiplate™ 96-Well PCR Plates, low profile, unskirted, clear

MLL9601

Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) Multiplate™ 96-Well PCR Plates, low profile, unskirted, white

MLL9651

Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001

Pkg of 100, optically clear seal for PCR plates

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) 0.2 ml 8-Tube PCR Strips without Caps, low profile, clear

TLS0801

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)

CFX96 Touch Deep Well 实时 PCR 检测系统 | Bio-Rad Laboratories

(停产) 0.2 ml 8-Tube PCR Strips without Caps, low profile, white

TLS0851

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)

(停产) 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear

TCS0803

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

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Precision Melt Analysis™ v1.2 Software Upgrade
SOFT-PM-12-PATCH
Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager™ Software version 2.0 and higher

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CFX Manager™ Software v3.0 Upgrade
SOFT-CFX-30-PATCH
Upgrade CFX Manager software from version 1.0–2.1 to version 3.0 for all CFX and MiniOpticon real-time PCR detection systems. If using CFX Manager software version 2.0 or lower, a firmware update is required for all CFX systems

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EXPORT COMPLIANCE REQUIREMENTS

Export of any Bio-Rad product or technical information relating to the product (i.e., manuals, memoranda, specifications, schematics, etc.) outside of the United States is subject to U.S. export control laws, including but not limited to the U.S. Export-Re-export Requirements and Enhanced Proliferation Control Initiative.

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

  1. use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
  2. sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
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All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

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