Students use western blotting to specifically identify myosin light chain from the hundreds of other proteins that comprise the muscle cell extracts of closely and distantly related species of fish.
描述
The Comparative Proteomics Kit II: Western Blot Module is the second part of a two-part laboratory. In the first part of this laboratory (Comparative Proteomics Kit I: Protein Profiler Module), students use sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to generate protein profiles and to visualize the unique array of proteins comprising muscle tissues from different fish. In this second part of the laboratory, students apply western blotting techniques to their polyacrylamide gel results to specifically identify myosin light chain from the hundreds of proteins that comprise the muscle cell extracts of closely and distantly related species of fish.
Features and Benefits
Applies immunology
Teaches students to use antibodies as detection tools
Serves as a laboratory extension for the Comparative Proteomics I Kit: Protein Profiler Module
How It Works
The protocol involves transferring the protein bands from polyacrylamide gels to a membrane and then using an anti-myosin light chain antibody to precisely identify which protein in each species’ profile is myosin light chain. Using Internet-based bioinformatics databases, students can compare their experimentally determined results to actual protein sequence data derived from DNA and RNA sequences and consider whether variations in myosin among species are due to “genetic” or “epigenetic” factors.
More Information
Four-session laboratory activity, 45 min per session
Provides sufficient materials for eight student workstations, up to four students per workstation
Related Products
Comparative Proteomics Kit I: Protein Profiler Module
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技术指标
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Curriculum, including teacher’s guide, student manual, and graphic quick guide
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(停产) Comparative Proteomics Kit II: Western Blot Module
1662800EDU
Western blot classroom study kit, includes antibodies, color detection reagents, 10x TG buffer, 10x PBS, 10% Tween, nitrocellulose, curriculum, and more, for 32 students; education use only
(停产) Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module
1662850EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU) and Western Blot Module (1662800EDU), for 32 students; education use only
(停产) Rapid Blotting and V3 Western Workflow Starter Kit
1662875EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU), Western Blot Module (1662800EDU), Trans-Blot® Turbo Mini Nitrocellulose Transfer Packs (10), 4–20% Mini-PROTEAN® TGX Stain-Free Gels (10), application note, for 32 students; education use only
Refills
(停产) Western Blot Temperature Sensitive Reagents Bag
1662801EDU
Consumables refill reagent kit for use with Comparative Proteomics Kit II: Western Blot Module (1662800EDU), includes antibodies and color detection reagents; education use only
200 μg, lyophilized, primary antibody (anti-myosin light chain antibody); education use only
(停产) Goat X Mouse-HRP
1721011EDU
2 ml, EIA-grade, enzyme-antibody conjugate; education use only
(停产) HRP Conjugate Substrate Kit
1706431EDU
Colorimetric detection for western blotting kit, includes premixed 4CN, hydrogen peroxide solutions, color development buffer; makes 1 L color development solution; education use only
(停产) Nitrocellulose/Filter Paper Pack
1662807EDU
0.45 µm, 7 x 8.4 cm, nitrocellulose membrane (8 sheets)/filter paper (16 sheets) sandwich; education use only
相关资料
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Number描述Options
M1662876EDU
Bio-Rad Explorer™ Rapid Blotting + V3 Western Workflow (Stain-Free Rapid Blotting) Application Note, Rev A
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10004531
Instruction Manual, Comparative Proteomics Kit II: Western Blot Module, Bio-Rad Explorer Rev E
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5478
Instruction Manual, Comparative Proteomics Kit II: Western Blot Module Quick Guide, Bio-Rad Explorer™, Rev A