伯乐pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

Teach the central dogma and genetic engineering using E. coli and a gene from the bioluminescent jellyfish Aequorea victoria. This classic pGLO kit follows the same procedure used by molecular biologists to create “designer proteins”.

描述

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

Note: This kit can be modified to accommodate individual workstations in socially-distanced classrooms.
Download instructions for modification.

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Bio-Rad’s pGLO Bacterial Transformation Kit is the classic kit for teaching the central dogma and the basics of genetic engineering. In this bacterial transformation lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria, which causes the bacteria to glow green under UV light. Explore the concept of DNA > RNA > Protein > Trait and the roles that external and internal factors play in gene regulation.

Features and Benefits

  • Hands-on genetic engineering — from handling plasmids to performing heat shock and plating the transformants, students perform the same transformation protocol used by scientists on a daily basis around the world
  • Visible, dramatic phenotype — bacteria transformed with pGLO glow a brilliant fluorescent green under UV light. Once students transform bacteria with pGLO, they never forget the central dogma of molecular biology: DNA > RNA > Protein > Trait — Green Fluorescence
  • Gene expression and gene regulation — bacteria transformed with pGLO show green fluorescence only when they are grown in the presence of arabinose. Use this experiment to illustrate that not all genes are expressed at the same time and that environmental factors control when gene expression takes place

Each pGLO Bacterial Transformation Kit:

  • Includes consumables and reagents for 8 student workstations, with up to 4 students per workstation
  • Supports lab activities designed for two 50-minute sessions

pGLO Resources for Teachers

  • pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

    pGLO Plasmid Map and Resources

    Learn more about how the pGLO plasmid system works, links to videos, activities, and more.

  • pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

    pGLO PowerPoint Presentation

    Download an editable, student-facing presentation for classroom use.

Find the kit instruction manual, student quick guide, and more under the Documents tab of this page.

 

Bio-Rad’s Family of pGLO Bacterial Transformation and GFP Kits

 

Bio-Rad offers a selection of kits for teaching about pGLO bacterial transformation, gene expression, and the green fluoresecent protein (GFP).

pGLO Bacterial Transformation Kits

A selection of pGLO bacterial transformation kits to help you meet your specific learning objectives

  pGLO Bacterial Transformation Kit pGLO Bacterial Transformation Kit for General Biology pGLO Bacterial Transformation and Inquiry Kit for AP Biology
Overview A laboratory skills-based approach to bacterial transformation; students follow a robust protocol for a well-controlled experiment while analyzing the details of the technique A highly scaffolded three-dimensional approach to bacterial transformation and gene expression; students design their own step-wise experiments and write and revise models and scientific explanations An inquiry-based approach to bacterial transformation through experimental optimization; students ask their own questions and conduct multiple experiments to refine the parameters of the technique
Primarily curriculum concepts
Gene expression, gene regulation, antibiotic resistance,
artificial selection, experimental design, laboratory skills
Workstations
8
12
8
Laboratory activities
1
2
5
Class periods
(50 min)
2
3–5
Flexible to fit a range of class periods

pGLO and Green Fluorescent Protein (GFP) Kits

pGLO extension activities for investigating the properties and purification of the green fluorescent protein (GFP)

  pGLO SDS-PAGE Extension* Green Fluorescent Protein Chromatography Kit* Secrets of the Rainforest Kit
Overview Use protein electrophoresis (SDS-PAGE) to view the expression of proteins in pGLO bacteria Use chromatography to purify glowing green fluorescent protein from pGLO bacteria (GFP)  Simulate the drug discovery process in your classroom with a glowing protein (GFP)
Primary curriculum concepts Protein expression, electrophoresis Protein expression, protein structure and function, chromatography Cloning and screening bacterial libraries, protein purification, chromatography, process and ethics of drug development
Workstations
8
8
8

* Requires purchase of a pGLO bacterial transformation kit.

技术指标

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 

Plasmid (pGLO), lyophilized
20 μg

E. coli strain HB101 K-12, lyophilized
1 vial

LB nutrient broth, sterile
10 ml

LB nutrient agar powder
20 g

Ampicillin, lyophilized
30 mg

Arabinose, lyophilized
600 mg

Sterile transformation solution (CaCl2)
15 ml

Petri dishes, 60 mm, sterile
40

Inoculation loops, sterile
80

Microcentrifuge tubes, 2.0 ml, sterile, color-coded
60

Disposable plastic transfer pipets
50

Foam micro test tube holders
8

UV pen light
1

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Use the filters below to refine results!

(停产) pGLO Bacterial Transformation Kit

1660003EDU

Gene expression analysis classroom kit, includes GFP encoding plasmid, nutrient broth/powder, petri dishes, UV pen light, and more, for 32 students; education use only, download free manual

Refills

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) Transformation Reagent Refill Kit

1660555EDU

Consumable reagents for pGLO Bacterial Transformation Kits, includes pGLO Plasmid, E. coli, nutrient broth, transformation solution, ampicillin, arabinose; education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) pGLO™ Plasmid

1660405EDU

20 μg, lyophilized DNA, plasmid encoding the Green Fluorescent Protein (GFP), for use with the pGLO Bacterial Transformation Kit (#166-0003EDU); education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) E. coli Strain HB101 K-12

1660408EDU

Lyophilized bacterial strain; education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) LB Nutrient Broth

1660421EDU

10 ml, sterile broth; education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) Ampicillin

1660407EDU

30 mg, lyophilized reagent, antibiotic for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) Arabinose

1660406EDU

600 mg, lyophilized reagent, gene expression activator for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) LB Agar Powder

1660600EDU

20 g, makes forty 60 mm agar plates; education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) Transformation Solution

1660409EDU

15 ml, sterile CaCl2 solution, for use with the pGLO™ Bacterial Transformation Kit (#166-0003EDU); education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) pGLO™ Transformation Lab Prep DVD

1660540EDU

Instructional DVD for use with pGLO Bacterial Transformation Kit (#166-0003EDU)

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) LB Nutrient Agar Powder

1660472EDU

500 g, makes one thousand
60 mm agar plates; education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) Petri Dishes

1660470EDU

Pkg of 500, 60 mm x 15 mm, sterile; education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) Inoculation Loops

1660471EDU

Pkg of 100, 10 μl, sterile; education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) Jellyfish Foam Floating Racks

1660479EDU

Pkg of 8, microcentrifuge tube rack, holds 12 tubes; education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) Disposable Plastic Transfer Pipets

1660474EDU

Pkg of 500, sterile; education use only

pGLO Bacterial Transformation Kit | Bio-Rad Laboratories

(停产) Colored 1.5 ml Microcentrifuge Tubes

1660473EDU

Pkg of 600, 6 colors; education use only

相关资料

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Number 描述 Options

LIT1660033EDU

Instructor and Student Guide, pGLO Bacterial Transformation Kit

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3052

pGLO Bacterial Transformation Kit, Biotechnology Explorer, Quick Guide

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7433

pGLO Plasmid Map and Sequence

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6672

Map of AP-Aligned Bio-Rad Kits with Essential Knowledge

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6673

Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories

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6605

Map of AP-Aligned Bio-Rad Kits with Learning Objectives

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6581

20 Questions to Master Inquiry – pGLO™ Bacterial Transformation Kit, Rev A

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5891

Refresh Kit Components — Large Class/Multiple Class Preparation Guide

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M1660023

Instructor Guide, Protein Electrophoresis of GFP: A pGLO Bacterial Transformation Kit Extension

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