伯乐DNA Barcoding Kits | Bio-Rad Laboratories

DNA Barcoding Kits | Bio-Rad Laboratories

Incorporate PCR, DNA sequencing, and bioinformatics to teach students how to genetically identify species of fish, mammals, insects, or fungi using a DNA barcode. 

描述

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Use Biotechnology to Investigate Your Local Biodiversity

With our DNA Barcoding Kits, students determine the species of fish, mammals, insects, or fungi based on the DNA sequence of standard reference gene (cytochrome c oxidase I (COI) or the internal transcribed spacer (ITS)). Students extract DNA from tissue, amplify the gene by PCR, verify PCR products by agarose gel electrophoresis, sequence the gene, and then use the sequence obtained and bioinformatics to genetically identify the species. The unique sequence produced for a given sample is considered to be its DNA barcode.

Kits and refill modules are available for the investigation of fish, mammalian, insect, and fungal samples.

Features and Benefits

With this kit, students are able to:

  • Perform DNA extraction, PCR amplification, and gel electrophoresis
  • Use DNA sequencing and bioinformatics to determine species identity
  • Conduct investigations in their area to examine biodiversity or see if marketplace substitution is occurring
  • Contribute to global initiatives for barcoding life

Background Information

DNA barcoding adds a level of genetic identification to species classification.

Taxonomists traditionally make species determinations based on their in-depth knowledge of species characteristics and environments in order to categorize them. The power of DNA research now allows us to add a level of identification based on the sequencing of a conserved gene. For example, the cytochrome c oxidase 1 (COI) gene is a mitochondrial gene found in fish, birds, butterflies, flies, and other animal groups. The internal transcribed spacer (ITS) is found in fungi. Subtle changes in these sequences result in species-specific identification or genetic barcodes. Other conserved genes are used for barcoding other groups of organisms.

This information is critical in helping to assess biodiversity and conservation efforts, as well as commercially, where food marketplaces can be regulated.

More DNA Barcoding Kit Information

Bio-Rad offers DNA barcoding kits for the identification of fish, mammalian, insect, and fungal samples. Each kit:

  • Provides sufficient materials for processing up to 16 samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation).
  • Contains a DNA extraction module and PCR module, each of which can also be purchased separately.

Barcoding kit bundles containing reagents for agarose electrophoresis are also available. 

DNA samples may be sequenced using any university or commercial DNA sequencing service (available in most regions)*. Refer to the instruction manual for sample processing requirements (sample cleanup, primers, etc.). Also, please contact the service provider you wish to use for their specific requirements and services. 

Although sequencing is required to identify the species of each sample, these kits may also be used without DNA sequencing to teach PCR and gel electrophoresis.

*The DNA Barcoding Sequencing Module (1665115EDU) has been discontinued. All previous purchases of the DNA Sequencing Module may be used/redeemed through June 30, 2023.

Additional DNA Barcoding Resources

  • Fish DNA Barcoding Quiz Game — a fun, Jeopardy-style quiz game that helps prepare your students for the DNA barcoding lab activities.
  • Download this Fish Flashcard activity for a fun way to introduce the Fish DNA barcoding lab activity to your class.

Related Products

  • T100 Thermal Cycler for the Classroom

  • Professional Adjustable-Volume Digital Micropipets

  • Mini-Sub Cell GT Systems

技术指标

Kit Contents
Each DNA Barcoding Kit includes one DNA Extraction Module and one PCR Module

DNA Extraction Module

Cell resuspension solution, 5 ml
1

Cell lysis solution, 5 ml
1

Neutralization solution, 5 ml
1

Wash buffer, 10 ml
1

Matrix, 5 ml
1

Spin filters with caps
20

Microcentrifuge tubes, 2 ml
250

PCR Module

PCR tubes, 0.2 ml
50

PCR master mix (Taq DNA polymerase, dNTPs, buffer), 1.2 ml
1

Fish primer mix (40x), 50 µl
1

COI-ITS PCR Control DNA
1

UView 6x Loading Dye and Stain
200 µl

PCR molecular weight ruler, 200 µl
1

Sterile water, 2.5 ml
1

Curriculum
Easy Start and Answer Guide; instructor and student guides are free downloads

Refills

DNA Barcoding Kits | Bio-Rad Laboratories

(停产) DNA Extraction Reagent Pack

1665105EDU

Reagents for chromosomal DNA extraction, includes resuspension, lysis, and neutralization solutions, wash buffer, matrix, spin filters, and 2.0 ml microtubes for up to 16 extractions; for educational use only

DNA Barcoding Kits | Bio-Rad Laboratories

(停产) Certified Molecular Biology Agarose

1613100EDU

25 g, general purpose agarose for nucleic acid electrophoresis, certified free of inhibitors, DNases, RNases; education use only

DNA Barcoding Kits | Bio-Rad Laboratories

(停产) 50x Tris/Acetic Acid/EDTA (TAE)

1660742EDU

100 ml, TAE nucleic acid electrophoresis buffer solution, pH 8.0; education use only

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Fish DNA Barcoding Dataset
SOFT-FISH-DNA-DATASET
Sequencing data generated in an online classroom to determine the species of fish samples

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Software Download

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EXPORT COMPLIANCE REQUIREMENTS

Export of any Bio-Rad product or technical information relating to the product (i.e., manuals, memoranda, specifications, schematics, etc.) outside of the United States is subject to U.S. export control laws, including but not limited to the U.S. Export-Re-export Requirements and Enhanced Proliferation Control Initiative.

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

  1. use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
  2. sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
  3. transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses; or
  4. transfer, export or re-export, directly or indirectly, any Bio-Rad product, technology or software (or any part thereof, or the product of such technology or software, or any process or service which is the product of such technology or software) to any person or entity identified by the U.S. Department of Commerce as a sanctioned and/or embargoed person or entity. (For a current list of all sanctioned and/or embargoed persons or entities, please consult the U.S. Department of Commerce).

All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

For more information regarding U.S. export laws and regulations, contact the U.S. Department of Commerce and the U.S. Department of the Treasury, Office of Foreign Assets Control.

I agree with the compliance requirements.
(You are required to agree in order to download the software.)

Please agree to the compliance requirements for downloading the software.

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Fungi DNA Barcoding Dataset
SOFT-FUNGI-DNA-DATASET

Sequencing data generated in an online classroom to determine the species of fungal samples

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Software Download

Sorry, an error has occurred. Please try again. If the problem persists, please call {0} for assistance.

EXPORT COMPLIANCE REQUIREMENTS

Export of any Bio-Rad product or technical information relating to the product (i.e., manuals, memoranda, specifications, schematics, etc.) outside of the United States is subject to U.S. export control laws, including but not limited to the U.S. Export-Re-export Requirements and Enhanced Proliferation Control Initiative.

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

  1. use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
  2. sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
  3. transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses; or
  4. transfer, export or re-export, directly or indirectly, any Bio-Rad product, technology or software (or any part thereof, or the product of such technology or software, or any process or service which is the product of such technology or software) to any person or entity identified by the U.S. Department of Commerce as a sanctioned and/or embargoed person or entity. (For a current list of all sanctioned and/or embargoed persons or entities, please consult the U.S. Department of Commerce).

All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

For more information regarding U.S. export laws and regulations, contact the U.S. Department of Commerce and the U.S. Department of the Treasury, Office of Foreign Assets Control.

I agree with the compliance requirements.
(You are required to agree in order to download the software.)

Please agree to the compliance requirements for downloading the software.

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Mammals and Insects DNA Barcoding Dataset
SOFT-MAMM-INSECT-DNA-DATASET

Sequencing data generated in an online classroom to determine the species of mammalian and insect samples

Download Software

Software Download

Sorry, an error has occurred. Please try again. If the problem persists, please call {0} for assistance.

EXPORT COMPLIANCE REQUIREMENTS

Export of any Bio-Rad product or technical information relating to the product (i.e., manuals, memoranda, specifications, schematics, etc.) outside of the United States is subject to U.S. export control laws, including but not limited to the U.S. Export-Re-export Requirements and Enhanced Proliferation Control Initiative.

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

  1. use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
  2. sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
  3. transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses; or
  4. transfer, export or re-export, directly or indirectly, any Bio-Rad product, technology or software (or any part thereof, or the product of such technology or software, or any process or service which is the product of such technology or software) to any person or entity identified by the U.S. Department of Commerce as a sanctioned and/or embargoed person or entity. (For a current list of all sanctioned and/or embargoed persons or entities, please consult the U.S. Department of Commerce).

All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

For more information regarding U.S. export laws and regulations, contact the U.S. Department of Commerce and the U.S. Department of the Treasury, Office of Foreign Assets Control.

I agree with the compliance requirements.
(You are required to agree in order to download the software.)

Please agree to the compliance requirements for downloading the software.

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相关资料

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Number 描述 Options

10000145585

DNA Barcoding Kit Instructor Guide

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10000145586

DNA Barcoding Kit Student Guide

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10000147118

DNA Barcoding Kit Bioinformatics Guide

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