Rabbit anti-RNF135 Polyclonal Antibody(C-term)别名宿主特异性反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 别名 |
RNF135抗体;E3 ubiquitin-protein ligase RNF135; 632-; RIG-I E3 ubiquitin ligase; REUL; RING finger protein 135; Riplet; RNF135
|
| 宿主 |
Rabbit
|
| 特异性 |
This RNF135 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375-401 amino acids from the C-terminal region of human RNF135.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:1000
|
| 分子量 |
Predicted molecular weight: 48kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
| 研究领域 |
Neurodegeneration;Others
|
| 靶标 | |
| 背景说明 |
Acts as an E2-dependent E3 ubiquitin-protein ligase, involved in innate immune defense against viruses. Ubiquitinates DDX58 and is required for full activation of the DDX58 signaling resulting in interferon beta production.
|
| 细胞定位 |
Cytoplasm.
|
| UniProt |
Q8IUD6
|
| 参考文献 | |
| 参考文献 |
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
You, F., et al. Nat. Immunol. 10(12):1300-1308(2009) Visser, R., et al. Am. J. Med. Genet. A 149A (4), 806-808 (2009) : Oshiumi, H., et al. J. Biol. Chem. 284(2):807-817(2009) Gao, D., et al. PLoS ONE 4 (6), E5760 (2009) : |
实验结果图
Anti-RNF135 Antibody (C-term) at 1:500 dilution + human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
RNF135 Antibody (C-term)(Cat. #AP19376b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RNF135 antibody detected the RNF135 protein (arrow).