anti-Flag Tag Mouse Monoclonal antibody(鼠单克隆抗体)M1001060

anti-Flag Tag Mouse Monoclonal antibody(鼠单克隆抗体)M1001060

产品名称:anti-Flag Tag Mouse Monoclonal antibody(鼠单克隆抗体)


产品特点:anti-Flag Tag Mouse Monoclonal antibody(鼠单克隆抗体)在Western Blotting中使用内参其实就是在WB过程中的另外用内参对应的抗体检测内参,这样在检测目的产物的同时可以检测内参的表达,由于内参在各组织和细胞中的表达相对恒定,借助检测每个样品内参的量就可以用于校正上样误差,这样半定量的结果才更为可信。

M1001060anti-Flag Tag Mouse Monoclonal antibody(鼠单克隆抗体)的详细资料:

英文名称      anti-Flag Tag Mouse Monoclonal antibody

应用             WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.

规  格           100ul

浓  度           1mg/ml

保  存           Store at -20°C. Do not aliquot the antibody.

储存液        PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

纯化方法     affinity purified by Protein A

亚  型           IgG

性  状           Liquid

克隆类型      monoclonal

宿  主           Mouse

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.