Mouse anti-IF4E Monoclonal Antibody(1504CT774.14.55)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
IF4E抗体;Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; EIF4E; EIF4EL1; EIF4F
|
| 宿主 |
Mouse
|
| 特异性 |
This IF4E antibody is generated from a mouse immunized with a recombinant protein of human IF4E.
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
IF~~1:25
WB~~1:4000 |
| 分子量 |
Predicted molecular weight: 25kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1504CT774.14.55
|
| 同种型 |
IgG1,Κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Crown Antibodies
|
| 靶标 | |
| 背景说明 |
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
|
| 细胞定位 |
Cytoplasm, P-body. Cytoplasm
|
| UniProt |
P06730
|
| 参考文献 | |
| 参考文献 |
Rychlik W.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:945-949(1987).
Rychlik W.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:1148-1148(1992). Ota T.,et al.Nat. Genet. 36:40-45(2004). Hillier L.W.,et al.Nature 434:724-731(2005). Oh J.H.,et al.Mamm. Genome 16:942-954(2005). |
实验结果图
All lanes : Anti-IF4E Antibody at 1:4000 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: K562 whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lane 5: PC-12 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.