Rabbit anti-LOK Polyclonal Antibody(C-term E707)描述别名宿主特异性反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Rabbit Polyclonal Antibody (Pab)
|
| 别名 |
LOK抗体;Serine/threonine-protein kinase 10; Lymphocyte-oriented kinase; STK10; LOK
|
| 宿主 |
Rabbit
|
| 特异性 |
This LOK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 692-721 amino acids from the C-terminal region of human LOK.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:1000
IHC-P~~1:50~100 |
| 分子量 |
Predicted molecular weight: 112kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Signal Transduction
|
| 靶标 | |
| 背景说明 |
Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.
|
| 细胞定位 |
Cell membrane; Peripheral membrane protein
|
| UniProt |
O94804
|
| 参考文献 | |
| 参考文献 |
Kuramochi, S., et al., Immunogenetics 49(5):369-375 (1999).
|
实验结果图
The anti-LOK Pab (Cat. #JP111446) is used in Western blot to detect LOK in Jurkat cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.