Lumiprobe-Cyanine5.5 NHS ester
CY5.5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。CY5.5属于是近红外荧光染料，当背景荧光有干扰时适宜用它来做标记以进行荧光分析。它也可以用于活体成像实验。该染料可以替代Alexa Fluor 680和DyLight 680。
During the last years, Cyanine5 (an analog of Cy5®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.
This Cyanine5 NHS ester (analog to Cy5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.
Cy® is a trademark of GE Healthcare.
|Appearance:||dark blue powder|
|Solubility:||soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water|
|Quality control:||NMR 1H (95%) and 13C, TLC, functional testing|
|Storage conditions:||Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.|
|Excitation maximum, nm:||673|
|Extinction coefficient at excitation maximum, Lmol-1cm-1:||209000|
|Emission maximum, nm:||707|
|Fluorescence quantum yield:||0.2|
|17020||Cyanine5.5 NHS ester, 1 mg|
|27020||Cyanine5.5 NHS ester, 5 mg|
|47020||Cyanine5.5 NHS ester, 25 mg|
|57020||Cyanine5.5 NHS ester, 50 mg|
|67020||Cyanine5.5 NHS ester, 100 mg|