Mouse anti-GARS Monoclonal Antibody(1641CT837.23.96)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
GARS抗体;Glycine–tRNA ligase; 6.1.1.14; Diadenosine tetraphosphate synthetase; AP-4-A synthetase; Glycyl-tRNA synthetase; GlyRS; GARS
|
| 宿主 |
Mouse
|
| 特异性 |
This GARS antibody is generated from a mouse immunized with a recombinant protein between 15-305 amino acids from human GARS.
|
| 反应种属 |
Human
|
| 应用 |
IF~~1:25
FC~~1:25 WB~~1:2000 |
| 分子量 |
Predicted molecular weight: 83kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1641CT837.23.96
|
| 同种型 |
IgG1,k
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
GARS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
|
| 靶标 | |
| 背景说明 |
Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
|
| 细胞定位 |
Cytoplasm. Mitochondrion
|
| UniProt |
P41250
|
| 参考文献 | |
| 参考文献 |
Shiba K.,et al.J. Biol. Chem. 269:30049-30055(1994).
Williams J.H.,et al.Nucleic Acids Res. 23:1307-1310(1995). Ota T.,et al.Nat. Genet. 36:40-45(2004). Hillier L.W.,et al.Nature 424:157-164(2003). Ge Q.,et al.J. Biol. Chem. 269:28790-28797(1994). |
实验结果图
All lanes : Anti-GARS Antibody at 1:4000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.