Purified Mouse Monoclonal Antibody (Mab)

SUMO2抗体, SUMO3抗体;Small ubiquitin-related modifier 3; SUMO-3; SMT3 homolog 1 {ECO:0000312|HGNC:HGNC:11124}; SUMO-2; Ubiquitin-like protein SMT3A; Smt3A; SUMO3

Mouse

Purified recombinant GST-SUMO3 fusion protein was used as immunogen.

Human

WB~~1:2000

Predicted molecular weight: 12kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Monoclonal Antibody

76AT630.91.31

Mouse IgG1

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Cell Biology

Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.

Cytoplasm. Nucleus. Nucleus, PML body

P55854

SENP3-mediated de-conjugation of SUMO2/3 from promyelocytic leukemia is correlated with accelerated cell proliferation under mild oxidative stress. Han Y, et al. J Biol Chem, 2010 Apr 23. PMID 20181954. Association of mitotic regulation pathway polymorphisms with pancreatic cancer risk and outcome. Couch FJ, et al. Cancer Epidemiol Biomarkers Prev, 2010 Jan. PMID 20056645. The p150 subunit of CAF-1 causes association of SUMO2/3 with the DNA replication foci. Uwada J, et al. Biochem Biophys Res Commun, 2010 Jan 1. PMID 19919826. SUMOylation of nuclear actin. Hofmann WA, et al. J Cell Biol, 2009 Jul 27. PMID 19635839. Heat shock protein 27 is involved in SUMO-2/3 modification of heat shock factor 1 and thereby modulates the transcription factor activity. Brunet Simioni M, et al. Oncogene, 2009 Sep 17. PMID 19597476.