Rabbit anti-MyD88 Polyclonal Antibody别名宿主特异性反应种属应用分子量免疫原浓度纯化方法类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt
| 概述 | |
| 别名 |
MyD88抗体;myeloid differential protein-88; Myeloid differentiation primary response gene; MYD 88; MYD88D; Myeloid differentiation marker 88; Myeloid differentiation primary response gene 88; Myeloid differentiation primary response protein MyD88; Myd88; MYD88_HUMAN; OTTHUMP00000161718; OTTHUMP00000208595; OTTHUMP00000209058; OTTHUMP00000209059; OTTHUMP00000209060
|
| 宿主 |
Rabbit
|
| 特异性 |
MyD88
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1ug/Test
|
| 分子量 |
Predicted molecular weight: 34kDa
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
KLH conjugated synthetic peptide derived from mouse MyD88:201-296/296
|
| 性能 | |
| 浓度 |
1mg/ml
|
| 纯化方法 |
affinity purified by Protein A
|
| 类型 |
Polyclonal Antibody
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
|
| 研究领域 |
细胞生物 免疫学 神经生物学
|
| 靶标 | |
| 背景说明 |
This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010].
|
| 细胞定位 |
细胞浆
|
| UniProt |
P22366
|
实验结果图
Sample: Molt-4 Cell (Human) Lysate at 40 ug
Primary: Anti-MyD88 (JP120585) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 36 kD
Paraformaldehyde-fixed, paraffin embedded (mouse kidney tissue); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (HO-1) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.