Mouse anti-NTRK2 Monoclonal Antibody(1446CT494.85.83.49)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
NTRK2抗体;BDNF/NT-3 growth factors receptor; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; NTRK2; TRKB
|
| 宿主 |
Mouse
|
| 特异性 |
This NTRK2 antibody is generated from a mouse immunized with a recombinant protein.
|
| 反应种属 |
Human
|
| 应用 |
IHC-P~~1:25
FC~~1:25 WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 92kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1446CT494.85.83.49
|
| 同种型 |
IgG1,k
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Cancer;Cell Biology;Developmental Biology;Metabolism;Neuroscience;Signal Transduction
|
| 靶标 | |
| 背景说明 |
Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin- 4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin- dependent calcium signaling in glial cells and mediate communication between neurons and glia.
|
| 细胞定位 |
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Note=Internalized to endosomes upon ligand-binding.
|
| UniProt |
Q16620
|
| 参考文献 | |
| 参考文献 |
Nakagawara A.,et al.Genomics 25:538-546(1995).
Shelton D.L.,et al.J. Neurosci. 15:477-491(1995). Allen S.J.,et al.Neuroscience 60:825-834(1994). Stoilov P.,et al.Biochem. Biophys. Res. Commun. 290:1054-1065(2002). Steinbeck J.A.,et al.Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases. |
实验结果图
Overlay histogram showing SH-SY5Y cells stained with JP100488 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (JP100488, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western blot analysis of lysate from human brain tissue lysate, using NTRK2 Antibody(Cat. #JP100488). JP100488 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20μg.