Rabbit anti-Phospho-mTOR(Ser2448) Monoclonal Antibody(7G7)别名宿主反应种属应用分子量免疫原形式纯化方法克隆号同种型储存/保存方法存储溶液修饰背景说明UniProt
| 概述 | |
| 别名 |
MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic tar
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse
|
| 应用 |
WB: 1/500-1/1000, IHC: 1/50-1/100
|
| 分子量 |
Detects a band of approximately 289 kD(Predicted molecular weight: 289 kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
A synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR
|
| 性能 | |
| 形式 |
Monoclonal Antibody
|
| 纯化方法 |
Affinity Purified
|
| 克隆号 |
7G7
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
|
| 修饰 |
Phosphorylated
|
| 靶标 | |
| 背景说明 |
An atypical kinase belonging to the PIKK family of kinases. Controls cell growth through protein synthesis regulation. Downstream of PI3K/Akt pathway and required for cell survival. Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex.
|
| UniProt |
P42345
|
实验结果图
Western blot analysis of Phospho-mTOR (Ser2448) in Hela, Hela+EGF lysates using Phospho-mTOR (Ser2448) antibody.
Western blot analysis of mTOR (Phospho-Ser2448) in 3t3 lysates using mTOR (Phospho-Ser2448) antibody.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Phospho-mTOR (Ser2448) antibodyHigh-pressure and temperature Sodium Citrate pH6.0 was used for antigen retrieval.