Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

ABL2抗体;Abelson tyrosine-protein kinase 2; Abelson murine leukemia viral oncogene homolog 2; Abelson-related gene protein; Tyrosine-protein kinase ARG; ABL2; ABLL; ARG

Rabbit

This ABL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 417-446 amino acids from human ABL2.

Human

M

WB~~1:1000

Predicted molecular weight: 128kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Polyclonal Antibody

Rabbit Ig

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Cancer;Signal Transduction

Non-receptor tyrosine-protein kinase that plays an ABL1- overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin- bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

Cytoplasm, cytoskeleton.

P42684

Cao, C., et al., Biochemistry 42(35):10348-10353 (2003).
Cao, C., et al., J. Biol. Chem. 278(32):29667-29675 (2003).
Kruh, G.D., et al., Proc. Natl. Acad. Sci. U.S.A. 87(15):5802-5806 (1990).
Kruh, G.D., et al., Science 234(4783):1545-1548 (1986).
Bianchi, C., et al., FEBS Lett. 527 (1-3), 216-222 (2002).