Rabbit anti-Phospho-Smad3(Ser423/425) Monoclonal Antibody(6A2)别名宿主反应种属应用分子量免疫原形式纯化方法克隆号同种型储存/保存方法存储溶液修饰背景说明UniProt
| 概述 | |
| 别名 |
SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Rat, Hamster
|
| 应用 |
WB: 1/500-1/1000
|
| 分子量 |
Detects a band of approximately 52 kD(Predicted molecular weight: 48 kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
A synthetic phosphopeptide corresponding to residues surrounding Ser423/Ser425 of human Smad3
|
| 性能 | |
| 形式 |
Monoclonal Antibody
|
| 纯化方法 |
Affinity Purified
|
| 克隆号 |
6A2
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
|
| 修饰 |
Phosphorylated
|
| 靶标 | |
| 背景说明 |
Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.
|
| UniProt |
P84022
|
实验结果图
Western blot analysis of Phospho-Smad3 (Ser423/425) in Jurkat, C6, CHOK1, Hela lysates using Phospho-Smad3 (Ser423/425) antibody.