Rabbit anti-Phospho-Src(Tyr419) Polyclonal Antibody别名宿主反应种属应用分子量免疫原形式纯化方法同种型储存/保存方法存储溶液修饰背景说明UniProt
| 概述 | |
| 别名 |
SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1/500-1/1000, FC: 1/50-1/100
|
| 分子量 |
Detects a band of approximately 60 kD(Predicted molecular weight: 60 kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
A synthesized peptide derived from human Phospho-Src (Y419)
|
| 性能 | |
| 形式 |
Polyclonal Antibody
|
| 纯化方法 |
Affinity Chromatography
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
|
| 修饰 |
Phosphorylated
|
| 靶标 | |
| 背景说明 |
Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
|
| UniProt |
P12931
|
实验结果图
Western blot analysis of Phospho-Src (Y419) in A431 lysates treated with pervanadate using Phospho-Src (Tyr419) antibody.