Rabbit anti-SLC7A5/LAT1 Recombinant Monoclonal Antibody(S-367-12)别名宿主特异性反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明组织特异性细胞定位UniProt
| 概述 | |
| 别名 |
SLC7A5抗体;Large neutral amino acids transporter small subunit 1; 4F2 light chain (4F2 LC; 4F2LC); CD98LC; MPE16; hLAT1; y+ system cationic amino acid transporter
|
| 宿主 |
Rabbit
|
| 特异性 |
SLC7A5 Antibody detects endogenous levels of total SLC7A5.
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:1000, IHC-P: 1:2000
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
S-367-12
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
SLC7A5 (Large neutral amino acids transporter small subunit 1), also known as 4F2 light chain, or CD98 light chain is a protein that in humans is encoded by the SLC7A5 gene. It is suggested to play a part in altered cell metabolism and proliferative signaling and has been reported to be overexpressed in various types of cancer, including breast cancer [PMID: 34792178].
|
| 组织特异性 |
Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.
|
| 细胞定位 |
Membrane, Lysosome
|
| UniProt |
Q01650
|
实验结果图
WB result of SLC7A5/LAT1 Rabbit mAb Primary antibody: SLC7A5/LAT1 Rabbit mAb at 1/1000 dilution Lane 1: LNCaP whole cell lysate 20 ug Lane 2: HT-29 whole cell lysate 20 ug Lane 3: HT-1080 whole cell lysate 20 ug Lane 4: MCF7 whole cell lysate 20 ug Negative control: LNCaP whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa Observed MW: 36 kDa Exposure time: 180 s
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.