Mouse anti-EIF4B Monoclonal Antibody(355CT12.6.5)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
EIF4B抗体;Eukaryotic translation initiation factor 4B; eIF-4B; EIF4B
|
| 宿主 |
Mouse
|
| 特异性 |
This EIF4B monoclonal antibody is generated from mouse immunized with EIF4B recombinant protein.
|
| 反应种属 |
Human
|
| 应用 |
WB~~ 1:500
|
| 分子量 |
Predicted molecular weight: 69kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
355CT12.6.5
|
| 同种型 |
IgG1,k
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Others
|
| 靶标 | |
| 背景说明 |
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5′- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
|
| UniProt |
P23588
|
| 参考文献 | |
| 参考文献 |
Mokas, S., et al. Mol. Biol. Cell 20(11):2673-2683(2009)
Rozovsky, N., et al. RNA 14(10):2136-2148(2008) Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007) Olsen, J.V., et al. Cell 127(3):635-648(2006) Olsen, J.V., et al. Cell 127(3):635-648(2006) |
实验结果图
EIF4B Antibody (Cat. #JP100160) western blot analysis in Jurkat cell line lysates (35μg/lane).This demonstrates the EIF4B antibody detected the EIF4B protein (arrow).