Rabbit anti-N-Cadherin Recombinant Monoclonal Antibody(329-12)描述别名宿主特异性反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明翻译后修饰细胞定位UniProt
| 概述 | |
| 描述 |
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density
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| 别名 |
N-Cadherin抗体;Cadherin-2; CDw325; Neural cadherin; CDH2; CDHN; NCAD
|
| 宿主 |
Rabbit
|
| 特异性 |
The antibody detects endogenous level of total N-Cadherin protein.
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1:1000, IHC-P: 1:500
|
| 免疫原 |
Synthetic Peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
329-12
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
The cell adhesion molecule (CAM), N-cadherin, has emerged as an important oncology therapeutic target. N-cadherin is a transmembrane glycoprotein mediating the formation and structural integrity of blood vessels. Its expression has also been documented in numerous types of poorly differentiated tumours [PMID: 25533096].
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| 翻译后修饰 |
Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).May be phosphorylated by OBSCN.
|
| 细胞定位 |
Cell membrane
|
| UniProt |
P19022
|
实验结果图
WB result of N-Cadherin Rabbit mAb Primary antibody: N-Cadherin Rabbit mAb at 1/1000 dilution Lane 1: MCF7 whole cell lysate 20 µg Lane 2: A549 whole cell lysate 20 µg Lane 3: HeLa whole cell lysate 20 µg Lane 4: PC-3 whole cell lysate 20 µg Lane 5: HepG2 whole cell lysate 20 µg Negative control: MCF7 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 100kDa Observed MW: 110~140kDa Exposure time: 60s
WB result of N-Cadherin Rabbit mAb Primary antibody: N-Cadherin Rabbit mAb at 1/1000 dilution Lane 1: mouse brain lysate 20 µg Lane 2: mouse heart lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 100kDa Observed MW: 140kDa Exposure time: 20s
WB result of N-Cadherin Rabbit mAb Primary antibody: N-Cadherin Rabbit mAb at 1/1000 dilution Lane 1: rat brain lysate 20 µg Lane 2: rat heart lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 100kDa Observed MW: 110~140kDa Exposure time: 180s
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative tissue: IHC shows negative staining in paraffin-embedded human chromophobe renal carcinoma. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.