Mouse anti-RAB27A Monoclonal Antibody(1590CT813.266.26)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
RAB27A抗体;Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram; RAB27A; RAB27
|
| 宿主 |
Mouse
|
| 特异性 |
This RAB27A antibody is generated from a mouse immunized with a recombinant protein of human RAB27A.
|
| 反应种属 |
Human
|
| 应用 |
FC~~1:25
WB~~1:2000 |
| 分子量 |
Predicted molecular weight: 25kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1590CT813.266.26
|
| 同种型 |
IgG1,k
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Signal Transduction
|
| 靶标 | |
| 背景说明 |
Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
|
| 细胞定位 |
Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles
|
| UniProt |
P51159
|
| 参考文献 | |
| 参考文献 |
Seabra M.C.,et al.J. Biol. Chem. 270:24420-24427(1995).
Seabra M.C.,et al.Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases. Chen D.,et al.Biochem. Mol. Med. 60:27-37(1997). Tolmachova T.,et al.Gene 239:109-116(1999). Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000). |
实验结果图
Anti-RAB27A Antibody at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.