Rabbit anti-CD63 Recombinant Monoclonal Antibody(230-3)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
Granulophysin; LAMP-3; Limp1; OMA81H; Tspan-30
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:500, IHC-P: 1:1000-1:2000
|
| 免疫原 |
Recombinant protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.25 mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
230-3
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
CD63 is a member of the tetraspanin superfamily, a cluster of cell surface associated membrane proteins with four transmembrane domains [PubMed: 31882993, PubMed: 25921073]. Tetraspanins have been shown to be involved in many cellular functions including cell motility, adhesion, differentiation,activation, immune response, and tumor cell migration and invasion. Post translational modification of CD63 results in its ability to organize and form tetraspanin-enriched mi_x0002_crodomains (TEMs) on membranes. The formation of TEMs allows interaction with components such as integrin molecules, immunoglobulins, proteoglycans, cadherins, and signaling molecules on the cell surface [PubMed: 31882993, PubMed: 15992681]. CD63 is highly enriched in late endosomal and lysosomal compartments after being endocytosed from the cell surface through the clathrin-coated vesicles.
|
| 细胞定位 |
Cell membrane, Lysosome membrane, Late endosome membrane, Melanosome, Secreted
|
| UniProt |
P08962
|
实验结果图
WB result of CD63 Rabbit mAb Primary antibody: CD63 Rabbit mAb at 1/500 dilution Lane 1: Jurkat whole cell lysate 20 µg Lane 2: SK-MEL-28 whole cell lysate 20 µg Lane 3: THP-1 whole cell lysate 20 µg Lane 4: 293T whole cell lysate 20 µg Negative control: Jurkat whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 25 kDa Observed MW: 25~60 kDa, 25~100 kDa Exposure time: 60s
IHC shows positive staining in paraffin-embedded human colon. Anti-CD63 antibody was used at 1/2000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD63 antibody was used at 1/2000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD63 antibody was used at 1/2000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD63 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CD63 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-CD63 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human melanoma. Anti-CD63 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.