Rabbit anti-ZNF217 Polyclonal Antibody(N-term)描述别名宿主特异性反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
|
| 别名 |
ZNF217抗体;Zinc finger protein 217; ZNF217; ZABC1
|
| 宿主 |
Rabbit
|
| 特异性 |
This ZNF217 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-120 amino acids from the N-terminal region of human ZNF217.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:1000
|
| 分子量 |
Predicted molecular weight: 115kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
| 研究领域 |
Cancer
|
| 靶标 | |
| 背景说明 |
Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at ‘Ser-473’.
|
| 细胞定位 |
Nucleus.
|
| UniProt |
O75362
|
| 参考文献 | |
| 参考文献 |
Krig, S.R., et al. Oncogene 29(40):5500-5510(2010)
Plevova, P., et al. Neoplasma 57(4):325-332(2010) Sun, G.Q., et al. Nan Fang Yi Ke Da Xue Xue Bao 29(4):685-688(2009) Banck, M.S., et al. Epigenetics 4(2):100-106(2009) Thillainadesan, G., et al. Mol. Cell. Biol. 28(19):6066-6077(2008) |
实验结果图
Anti-ZNF217 Antibody (N-term)at 1:2000 dilution + MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
ZNF217 Antibody (N-term) (Cat. #JP102692) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ZNF217 antibody detected the ZNF217 protein (arrow).