Rabbit anti-PSA Recombinant Monoclonal Antibody(195-14)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
Prostate-specific antigen; Seminin; Kallikrein-3; P-30 antigen; Semenogelase
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:10000, IHC-P: 1:1000
|
| 免疫原 |
Recombinant protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.25 mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
195-14
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Prostate-specific antigen (PSA) is a biomarker for the diagnosis and management of prostate cancer and involved in the development of prostate cancer and/or its progression from the localized to the metastatic stage.
|
| 细胞定位 |
Secreted
|
| UniProt |
P07288
|
实验结果图
WB result of PSA Rabbit mAb Primary antibody: PSA Rabbit mAb at 1/10000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: LNCaP whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 34 kDa Observed MW: 34 kDa Exposure time: 60s
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-PSA antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate cancer. Anti-PSA antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-PSA antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human ovarian cancer. Anti-PSA antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.