Rabbit anti-ERG Recombinant Monoclonal Antibody(190-65)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
Transcriptional regulator ERG; Transforming protein ERG
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:500, IHC-P: 1:1000
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.25 mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
190-65
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
ERG is an ETS family transcription factor (TF) frequently involved in human cancers, including leukemia [PMID: 21664289], prostate cancer [PMID: 16254181] and Ewing’s sarcoma [PMID: 8223458, PMID: 10561219]. It functions as an oncogene through chromosomal translocations or overexpression. In human leukemia, ERG was initially thought to play a role in leukemogenesis based on the t(16;21) translocation in acute myeloid leukemia (AML), leading to formation of the FUS (TLS)-ERG fusion [PMID: 8234289]. In some cases of AML with this translocation, the leukemia exhibits features of acute megakaryoblastic leukemia [PMID: 12091356].
|
| 细胞定位 |
Nucleus, Cytoplasm
|
| UniProt |
P11308
|
实验结果图
WB result of ERG Rabbit mAb Primary antibody: ERG Rabbit mAb at 1/500 dilution Lane 1: Jurkat whole cell lysate 20 µg Lane 2: Hela whole cell lysate 20 µg Lane 3: MOLT-4 whole cell lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 41~55 kDa Observed MW: 41~55 kDa Exposure time: 180s
IHC shows positive staining in paraffin-embedded human kidney. Anti-ERG antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-ERG antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ERG antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ERG antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.