Rabbit anti-Smad4 Recombinant Monoclonal Antibody(168-67)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
DPC4
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1:1000
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
liquid
|
| 浓度 |
0.5 mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal antibody
|
| 克隆号 |
168-67
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
SMAD (mothers against decapentaplegic homologs) molecules are the core components in TGF-β signaling pathway. TGF-β binding to its receptor induces phosphorylation and activation of receptor-regulated SMADs (R-SMADs), SMAD2 and SMAD3, which subsequently associate with their partner SMAD4 and translocate from cytoplasm to nucleus. Formation of R-SMAD–SMAD4 complexes is essential in signaling of most TGF-β family members
|
| 细胞定位 |
Cytoplasm, Nucleus
|
| UniProt |
Q13485
|
实验结果图
WB result of Smad4 Rabbit mAb Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution Lane 1: SW480 whole cell lysate 20 µg Lane 2: HT-29 whole cell lysate 20 µg Lane 3: HeLa whole cell lysate 20 µg Lane 4: HepG2 whole cell lysate 20 µg Lane 5: Jurkat whole cell lysate 20 µg Negative control: SW480 whole cell lysate; HT-29 whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 70 kDa Observed MW: 65 kDa Exposure time: 180s
WB result of Smad4 Rabbit mAb Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution Lane 1: NIH/3T3 whole cell lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 70 kDa Observed MW: 65 kDa Exposure time: 180s
WB result of Smad4 Rabbit mAb Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution Lane 1: C6 whole cell lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 70 kDa Observed MW: 65 kDa Exposure time: 180s