Rabbit anti-CD166 Polyclonal Antibody别名宿主特异性反应种属预测反应种属应用分子量免疫原浓度纯化方法类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt
| 概述 | |
| 别名 |
CD166抗体;activated leukocyte cell adhesion molecule; ALCAM protein; ALCAM; CD 166; CD166 antigen; FLJ38514; MEMD; MGC71733; FLJ3851; AI853494; BEN; DM-GRASP; MGC27910; MuSC; SC1; CD166_HUMAN
|
| 宿主 |
Rabbit
|
| 特异性 |
CD166
|
| 反应种属 |
Human,
|
| 预测反应种属 |
Mouse;Rat;Chicken;Dog;Pig;Cow;Horse;Rabbit;
|
| 应用 |
WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
|
| 分子量 |
Predicted molecular weight: 62kDa
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
KLH conjugated synthetic peptide derived from human CD166:451-583/583
|
| 性能 | |
| 浓度 |
1mg/ml
|
| 纯化方法 |
affinity purified by Protein A
|
| 类型 |
Polyclonal Antibody
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
|
| 研究领域 |
肿瘤 细胞粘附分子 细胞表面分子
|
| 靶标 | |
| 背景说明 |
CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for CD166.
The expression of CD166 is up-regulated in low-grade prostate tumors and down-regulated in high-grade tumors; may play role in progression of prostate cancer. |
| 细胞定位 |
细胞膜
|
| UniProt |
Q13740
|
实验结果图
Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-CD166 (JP122089) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD
Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-CD166 (JP122089) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD
Blank control: Molt-4 Cells(blue).
Primary Antibody: Rabbit Anti-CD166/FITC Conjugated antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed.