Rabbit anti-p40 Recombinant Monoclonal Antibody(166-36)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
BC28
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:1000, IHC-P: 1:500
|
| 免疫原 |
Synthetic Peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
166-36
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
p63 (TAp63) is closely related to p40 (ΔNp63) as both proteins represent isoforms of the p63 gene with distinct molecular functions. While “full length” p63 (TAp63) activates p53 target genes such as p21 or BAX, the shorter transcript p40 (ΔNp63) inhibits activation of p53 and “full length” p63. Among carcinomas, p40 has approximately the same sensitivity as p63 but a higher specificity, as the TAp63 isoform is expressed more widespread in eg., adenocarcinomas. Moreover, p63 occur in lymphomas that are p40 negative.
|
| 细胞定位 |
Nucleus
|
| UniProt |
Q9H3D4-4
|
实验结果图
WB result of p40 Rabbit mAb Primary antibody: p40 Rabbit mAb at 1/1000 dilution Lane 1: MCF7 whole cell lysate 20 ug Lane 2: A431 whole cell lysate 20 ug Lane 3: HaCaT whole cell lysate 20 ug Negative control: MCF7 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51 kDa Observed MW: 51, 75 kDa Exposure time: 180 s
IHC shows positive staining in paraffin-embedded human tonsil. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.