Rabbit anti-CD163 Polyclonal Antibody别名宿主特异性反应种属预测反应种属应用分子量免疫原浓度纯化方法类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt
| 概述 | |
| 别名 |
CD163抗体, M130抗体;Scavenger receptor cysteine-rich type 1 protein M130; CD 163; CD163 antigen; CD163 molecule; Hemoglobin Scavenger Receptor; M130; M130 antigen precursor; Macrophage associated antigen; MM130; C163A_HUMAN
|
| 宿主 |
Rabbit
|
| 特异性 |
CD163
|
| 反应种属 |
Human, Mouse
|
| 预测反应种属 |
Rat;Dog;Pig;Horse;
|
| 应用 |
Flow-Cyt=1ug/Test
|
| 分子量 |
Predicted molecular weight: 130kDa
|
| 免疫原 |
KLH conjugated synthetic peptide derived from human CD163:1001-1121/1156
|
| 性能 | |
| 浓度 |
1mg/ml
|
| 纯化方法 |
affinity purified by Protein A
|
| 类型 |
Polyclonal Antibody
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
|
| 研究领域 |
心血管 免疫学 细胞膜受体
|
| 靶标 | |
| 背景说明 |
CD163 is a 130 kDa membrane glycoprotein. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.
|
| 细胞定位 |
细胞膜 分泌型蛋白
|
| UniProt |
Q86VB7
|
实验结果图
Blank control (Black line): U2OS.
Primary antibody: Rabbit Anti-CD163 antibody
Dilution: 3μg /10^6 cells;
Isotype Control antibody: Rabbit IgG .
Secondary antibody: Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. Cells incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.