Purified Mouse Monoclonal Antibody (Mab)

CDK4抗体;Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3; CDK4

Mouse

This CDK4 antibody is generated from a mouse immunized with a recombinant protein.

Human, Mouse

IHC-P~~1:25
WB~~1:2000

Predicted molecular weight: 34kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Monoclonal Antibody

1529CT850.162.73

IgG2a,κ

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cancer;Cell Biology

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Cytoplasm. Nucleus. Membrane. Note=Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus

P11802

Hanks S.K.,et al.Submitted (FEB-1987) to the EMBL/GenBank/DDBJ databases.
Elkahloun A.G.,et al.Genomics 42:295-301(1997).
Wolfel T.,et al.Science 269:1281-1284(1995).
Zuo L.,et al.Nat. Genet. 12:97-99(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).