Mouse anti-EEF1E1 Monoclonal Antibody(1494CT639.147.31)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
EEF1E1抗体;Eukaryotic translation elongation factor 1 epsilon-1; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; Elongation factor p18; Multisynthase complex auxiliary component p18; EEF1E1; AIMP3; P18
|
| 宿主 |
Mouse
|
| 特异性 |
This EEF1E1 antibody is generated from a mouse immunized with a recombinant protein of human EEF1E1.
|
| 反应种属 |
Human, Mouse
|
| 应用 |
IF~~1:25
FC~~1:25 WB~~1:2000-1:4000 |
| 分子量 |
Predicted molecular weight: 20kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1494CT639.147.31
|
| 同种型 |
IgG2b,κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Cell Biology
|
| 靶标 | |
| 背景说明 |
Positive modulator of ATM response to DNA damage.
|
| 细胞定位 |
Cytoplasm. Nucleus. Note=Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage
|
| UniProt |
O43324
|
| 参考文献 | |
| 参考文献 |
Motegi H.,et al.Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Mao M.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998). Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. Mungall A.J.,et al.Nature 425:805-811(2003). Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB. |
实验结果图
Overlay histogram showing Hela cells stained with JP100503 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (JP100503, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-EEF1E1 Antibody at 1:2000-1:4000 dilution Lane 1: HepG2 whole cell lysates Lane 2: human testis lysates Lane 3: A549 whole cell lysates Lane 4: A431 whole cell lysates Lane 5: Jurkat whole cell lysates Lane 6: mouse brain lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.