Rabbit anti-CD40 Recombinant Monoclonal Antibody(145-45)别名宿主反应种属应用分子量免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:1000, IP: 1:25, IHC-P: 1:100-500, FC (Extra)：1:500
|
| 分子量 |
42kDa
|
| 免疫原 |
recombinant protein
|
| 性能 | |
| 形式 |
liquid
|
| 浓度 |
0.5mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal antibody
|
| 克隆号 |
145-45
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
The CD40 is a type I transgender protein that is transmitted in the immune system’s B cells and special antigen to the surface of the cells and many non -hematopoietic cell types and cancer cells. It belongs to the TNF receptor super family.CD40 is expressed in B cells, foam dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines. CD40 interacts with CD40 Ligand (CD40L/TNFSF5).CD40/CD40L is essential for the beginning and progress of cells and body fluid adaptive immunity, and it is an important area of concern in the study of tumor immunology, neurodegeneration, vascular disease and inflammatory diseases.
|
| 细胞定位 |
Cell membrane, Secreted
|
| UniProt |
P25942
|
实验结果图
WB result of CD40 Rabbit mAb Primary antibody:CD40 Rabbit mAb at 1/1000 dilution Lane 1: Jurkat whole cell lysate 20 µg Lane 2: Raji whole cell lysate 20 µg Lane 3: Ramos whole cell lysate 20 µg Lane 4: Daudi whole cell lysate 20 µg Negative control: Jurkat whole cell lysate Secondary antibody:#JP20040 at 1/10000 dilution Predicted MW: 42 kDa Observed MW: 39 kDa Exposure time: 40s
Flow cytometric analysis of Jurkat (left) / Ramos (right) cells labelling CD40 antibody at 1/500 dilution (0.1ug)/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody: #JP20025 was used as the secondary antibody. Negative control: Jurkat
IHC shows positive staining in paraffin-embedded human tonsil.Anti-CD40 antibody was used at 1/100 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen.Anti-CD40 antibody was used at 1/100 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma.Anti-CD40 antibody was used at 1/500 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cancer.Anti-CD40 antibody was used at 1/100 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
CD40 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating CD40 in 0.4mg Raji whole cell lysate. Western blot was performed on the immunoprecipitate using CD40 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: Raji whole cell lysate 10µg (input) Lane 2 (+): CD40 Rabbit mAb IP in Raji whole cell lysate Lane 3 (-): Rabbit monoclonal IgG IP in Raji whole cell lysate Predicted MW: 42 kDa Observed MW: 39 kDa Exposure time: 50s