Rabbit anti-RNF139 Polyclonal Antibody(C-Term)描述别名宿主特异性反应种属应用分子量类型同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Rabbit Polyclonal Antibody (Pab)
|
| 别名 |
RNF139抗体;E3 ubiquitin-protein ligase RNF139; 632-; RING finger protein 139; Translocation in renal carcinoma on chromosome 8 protein; RNF139
|
| 宿主 |
Rabbit
|
| 特异性 |
This RNF139 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 604-636 amino acids from human RNF139.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:2000
|
| 分子量 |
Predicted molecular weight: 76kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Cancer;Cell Biology
|
| 靶标 | |
| 背景说明 |
E3-ubiquitin ligase; acts as a negative regulator of the cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP/SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination. May function as a signaling receptor.
|
| 细胞定位 |
Endoplasmic reticulum membrane; Multi-pass membrane protein
|
| UniProt |
Q8WU17
|
| 参考文献 | |
| 参考文献 |
Gemmill R.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:9572-9577(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Lorick K.L.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:11364-11369(1999). Gemmill R.M.,et al.Oncogene 21:3507-3516(2002). |
实验结果图
All lanes : Anti-RNF139 Antibody (C-Term) at 1:2000 dilution Lane 1: Caki-1 whole cell lysate Lane 2: human brain lysate Lane 3: human heart lysate Lane 4: human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.