Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

RNF128抗体;E3 ubiquitin-protein ligase RNF128; 632-; Gene related to anergy in lymphocytes protein; GRAIL; RING finger protein 128; RNF128

Rabbit

This RNF128 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the Central region of human RNF128.

Human

B

WB~~1:1000

Predicted molecular weight: 47kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Greater than 95% as determined by reducing SDS-PAGE.

Polyclonal Antibody

Rabbit Ig

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Cell Biology

E3 ubiquitin-protein ligase that catalyzes ‘Lys-48’- and ‘Lys-63’-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with ‘Lys- 48’ linkages and COR1A with ‘Lys-63’ linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.

Endomembrane system; Single- pass membrane protein. Cytoplasm, cytoskeleton Cytoplasm, perinuclear region. Note=Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A (By similarity).

Q8TEB7

Su, L.L., et al. J. Immunol. 183(1):438-444(2009)
Lin, J.T., et al. J. Immunol. 182(10):5919-5928(2009)
Lineberry, N., et al. J. Biol. Chem. 283(42):28497-28505(2008)
Egawa, S., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 295 (1), G163-G169 (2008) :
Kostianovsky, A.M., et al. J. Immunol. 178(10):6158-6163(2007)