Mouse anti-CD74 Monoclonal Antibody(1267CT820.116.140.154)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
CD74抗体;HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CD74; DHLAG
|
| 宿主 |
Mouse
|
| 特异性 |
This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-232 amino acids from human.
|
| 反应种属 |
Human
|
| 应用 |
FC~~1:25
WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 34kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1267CT820.116.140.154
|
| 同种型 |
IgG2b,κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Immunology
|
| 靶标 | |
| 背景说明 |
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
|
| 细胞定位 |
Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome Lysosome. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane
|
| UniProt |
P04233
|
| 参考文献 | |
| 参考文献 |
Claesson L.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:7395-7399(1983).
Strubin M.,et al.EMBO J. 3:869-872(1984). Kudo J.,et al.Nucleic Acids Res. 13:8827-8841(1985). O’Sullivan D.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:4484-4488(1986). Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. |
实验结果图
Flow cytometric analysis of Raji cells using CD74(green, Cat#JP100411) compared to an isotype control of mouse IgG2b(blue). JP100411 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from Daudi, Raji cell line (from left to right), using CD74 Antibody(Cat. # JP100411). JP100411 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35μg per lane.