Rabbit anti-Desmin Recombiant Monoclonal Antibody(109-67)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
DES
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1:500, IHC:1:1000
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
liquid
|
| 浓度 |
0.25mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal antibody
|
| 克隆号 |
109-67
|
| 储存/保存方法 |
Store at- 20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Desmin is a 53 kDa muscle -specific molecule, which belongs to the middle silk super family of cell skeleton protein. Cyclical protein digosum formation forms a stable wire -shaped network, which can stabilize the horizontal arrangement of the muscle fiber. Most mutations affect filament assembly. Desmin knockout mice will form myocardial disease, skeletal muscle defects and smooth muscle defects. It is a good screening symbol of tumor with muscle -derived differentiation, including transverse muscle sarcoma, horizontal fibroids, smooth muscle sarcoma, smooth fibroids, smooth muscles, and horizontal muscle cells in other tumors.
|
| 细胞定位 |
Cell membrane, Cytoplasm, Intermediate filament,Nucleus
|
| UniProt |
P17661
|
实验结果图
WB result of Desmin Rabbit mAb Primary antibody: Desmin Rabbit mAb at 1/500 dilution Lane 1: Hela whole cell lysate 20 µg Lane 2: A431 whole cell lysate 20 µg Lane 3: C2C12 whole cell lysate 20 µg Negative control: Hela whole cell lysate Low expression control: A431 whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 53 kDa Observed MW: 53 kDa Exposure time: 30s
WB result of Desmin Rabbit mAb Primary antibody: Desmin Rabbit mAb at 1/500 dilution Lane 1: mouse heart lysate 20 µgSecondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 53 kDa Observed MW: 53 kDa Exposure time: 120s
WB result of Desmin Rabbit mAb Primary antibody: Desmin Rabbit mAb at 1/500 dilution Lane 1: rat heart whole cell lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 53 kDa Observed MW: 53 kDa Exposure time: 30s
IHC shows positive staining in paraffin-embedded human heart. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle.Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human brain. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human GIST. Anti-Desmin antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.