Mouse anti-YWHAZ Monoclonal Antibody(1314CT423.108.153.173.140)描述别名宿主特异性反应种属预测反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
YWHAZ抗体;14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ
|
| 宿主 |
Mouse
|
| 特异性 |
This YWHAZ antibody is generated from a mouse immunized with a recombinant protein from human YWHAZ.
|
| 反应种属 |
Human, Mouse, Rat
|
| 预测反应种属 |
Bovine
|
| 应用 |
IF~~1:25
WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 28kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1314CT423.108.153.173.140
|
| 同种型 |
IgG2b,κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long time storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Cell Biology;Neuroscience;Signal Transduction
|
| 靶标 | |
| 背景说明 |
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
|
| 细胞定位 |
Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes
|
| UniProt |
P63104
|
| 参考文献 | |
| 参考文献 |
Zupan L.A.,et al.J. Biol. Chem. 267:8707-8710(1992).
Seluja G.A.,et al.Biochim. Biophys. Acta 1395:281-287(1998). Ota T.,et al.Nat. Genet. 36:40-45(2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003). |
实验结果图
Fluorescent image of U251 cells stained with YWHAZ Antibody (Cat#JP100410). JP100410 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from A431, HepG2, mouse NIH/3T3, rat PC-12 and C6 cell line, mouse brain tissue (from left to right), using YWHAZ Antibody (Cat. #JP100410). JP100410 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35μg per lane.