Purified Mouse Monoclonal Antibody (Mab)

RAC1抗体;Ras-related C3 botulinum toxin substrate 1; Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1; RAC1; TC25

Mouse

This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human.

Human, Rat

Bovine, Mouse

IHC-P~~1:25
FC~~1:100
WB~~1:1000

Predicted molecular weight: 21kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Monoclonal Antibody

1301CT276.121.104

IgG2b

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cancer;Cell Biology;Immunology;Signal Transduction;Amyotrophic Lateral Sclerosis ALS

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.

Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Cytoplasm. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity).

P63000

Didsbury J.,et al.J. Biol. Chem. 264:16378-16382(1989).
Drivas G.T.,et al.Mol. Cell. Biol. 10:1793-1798(1990).
Matos P.,et al.Biochem. Biophys. Res. Commun. 277:741-751(2000).
Jordan P.,et al.Oncogene 18:6835-6839(1999).
Schnelzer A.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.