Mouse anti-MBD3 Monoclonal Antibody(C-term)(995CT3.2.2)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
MBD3抗体;Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3; MBD3
|
| 宿主 |
Mouse
|
| 特异性 |
Purified His-tagged MBD3 protein was used to produced this monoclonal antibody.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:1000
|
| 分子量 |
Predicted molecular weight: 33kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
995CT3.2.2
|
| 同种型 |
IgG1
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Crown Antibodies
|
| 靶标 | |
| 背景说明 |
Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
|
| 细胞定位 |
Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes
|
| UniProt |
O95983
|
| 参考文献 | |
| 参考文献 |
Hendrich B., et al. Mol. Cell. Biol. 18:6538-6547(1998).
Ota T., et al. Nat. Genet. 36:40-45(2004). Grimwood J., et al. Nature 428:529-535(2004). Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Tatematsu K., et al. Genes Cells 5:677-688(2000). |
实验结果图
MBD3 Antibody (C-term) (Cat. #JP100350) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the MBD3 antibody detected the MBD3 protein (arrow).