Rabbit anti-EphB6 Polyclonal Antibody(C-term H990)描述别名宿主特异性反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Rabbit Polyclonal Antibody (Pab)
|
| 别名 |
EphB6抗体;Ephrin type-B receptor 6; HEP; Tyrosine-protein kinase-defective receptor EPH-6; EPHB6
|
| 宿主 |
Rabbit
|
| 特异性 |
This EphB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 990-1021 amino acids from the C-terminal region of human EphB6.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:1000
IHC-P~~1:50~100 |
| 分子量 |
Predicted molecular weight: 111kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Neuroscience
|
| 靶标 | |
| 背景说明 |
Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.
|
| 细胞定位 |
Membrane; Single-pass type I membrane protein
|
| UniProt |
O15197
|
| 参考文献 | |
| 参考文献 |
Freywald, A., et al., J. Biol. Chem. 278(12):10150-10156 (2003). Luo, H., et al., J. Clin. Invest. 110(8):1141-1150 (2002). Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). Luo, H., et al., J. Immunol. 167(3):1362-1370 (2001). Tang, X.X., et al., Proc. Natl. Acad. Sci. U.S.A. 97(20):10936-10941 (2000).
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实验结果图
Western blot analysis of anti-EphB6 C-term Pab (Cat. #JP111236) in Jurkat cell lysate. EphB6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.