Rabbit anti-PERK(pT982) Polyclonal Antibody描述别名宿主特异性反应种属应用分子量免疫原纯度纯化方法类型储存/保存方法UniProt
| 概述 | |
| 描述 |
Rabbit polyclonal antibody to PERK (pT982)
|
| 别名 |
PERK抗体;PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK
|
| 宿主 |
Rabbit
|
| 特异性 |
Recognizes endogenous levels of PERK (pT982) protein.
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB 1:500 – 1:1000, IHC 1:50 – 1:200
|
| 分子量 |
Detects a band of approximately 140kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PERK. The exact sequence is proprietary.
|
| 性能 | |
| 纯度 |
> 96 % by SDS-PAGE and HPLC analyses.
|
| 纯化方法 |
The antibody was purified by immunogen affinity chromatography.
|
| 类型 |
Polyclonal Antibody
|
| 储存/保存方法 |
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
|
| 靶标 | |
| UniProt |
Q9NZJ5
|
实验结果图
Western blot analysis of PERK (pT982) expression in A375 (A), MCF7 (B), mouse testis (C) whole cell lysates.
Immunohistochemical analysis of PERK (pT982) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.