Rabbit anti-Phospho-Rb2 p130(Ser952) Monoclonal Antibody(5D6)别名宿主反应种属应用分子量免疫原形式纯化方法克隆号同种型储存/保存方法存储溶液修饰背景说明UniProt
| 概述 | |
| 别名 |
Rb2; P130
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1/500-1/1000, IHC: 1/50-1/100, IF: 1/50-1/200
|
| 分子量 |
Detects a band of approximately 128 kD(Predicted molecular weight: 128 kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
A synthetic phosphopeptide corresponding to residues surrounding Ser952 of human Rb2 p130
|
| 性能 | |
| 形式 |
Monoclonal Antibody
|
| 纯化方法 |
Affinity Purified
|
| 克隆号 |
5D6
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
|
| 修饰 |
Phosphorylated
|
| 靶标 | |
| 背景说明 |
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 ‘Lys-20’ trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
|
| UniProt |
Q08999
|
实验结果图
Western blot analysis of Rb2 p130 (Phospho-Ser952) in K562, Hela lysates using Phospho-Rb2 p130 (Ser952) antibody.
Immunocytochemistry analysis of Rb2 p130 (Phospho-Ser952)(green) in Hela using Rb2 p130 (Phospho-Ser952) antibody, and DAPI(blue)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rb2 p130 (Phospho-Ser952) antibody.High-pressure and temperature Sodium Citrate pH6.0 was used for antigen retrieval.