Mouse Monoclonal Antibody (Mab)

GOLPH3抗体;Golgi phosphoprotein 3; Coat protein GPP34; Mitochondrial DNA JPence factor; MIDAS; GOLPH3; GPP34

Mouse

Purified His-tagged GOLPH3 protein was used to produced this monoclonal antibody.

Human

WB~~1:1000

Predicted molecular weight: 34kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Monoclonal Antibody

905CT9.1.1

IgG1

Maintain refrigerated at 2-8°C for up to 2 weeks. For long time storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Signal Transduction

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion intermembrane space. Cell membrane. Endosome. Note=Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes.

Q9H4A6

Bell A.W., et al. J. Biol. Chem. 276:5152-5165(2001).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Gregory S.G., et al. Nature 441:315-321(2006).
Bechtel S., et al. BMC Genomics 8:399-399(2007).
Nakashima-Kamimura N., et al. J. Cell Sci. 118:5357-5367(2005).