Mouse anti-Creatine Kinase BB Monoclonal Antibody(Center)(893CT29.1.1)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
Creatine Kinase BB抗体;Creatine kinase B-type; B-CK; Creatine kinase B chain; CKB; CKBB
|
| 宿主 |
Mouse
|
| 特异性 |
Purified His-tagged CKB protein was used to produced this monoclonal antibody.
|
| 反应种属 |
Human, Mouse
|
| 应用 |
WB~~1:1000
|
| 分子量 |
Predicted molecular weight: 43kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
893CT29.1.1
|
| 同种型 |
IgG1
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Cancer;Cardiovascular;Cell Biology;Metabolism;Neuroscience;Signal Transduction
|
| 靶标 | |
| 背景说明 |
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
|
| 细胞定位 |
Cytoplasm.
|
| UniProt |
P12277
|
| 参考文献 | |
| 参考文献 |
Villarreal-Levy G., et al. Biochem. Biophys. Res. Commun. 144:1116-1127(1987).
Mariman E.C.M., et al. Genomics 1:126-137(1987). Kaye F.J., et al. J. Clin. Invest. 79:1412-1420(1987). Mariman E.C.M., et al. Nucleic Acids Res. 17:6385-6385(1989). Ota T., et al. Nat. Genet. 36:40-45(2004). |
实验结果图
Creatine Kinase BB (CKB) Antibody (Center) (Cat. #JP100344) western blot analysis in MDA-MB453,293,Y79 cell line ,mouse stomach and brain tissue lysates (35μg/lane).This demonstrates the CKB antibody detected the CKB protein (arrow).